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Comparative Proteomic Analyses of Streptococcus suis Serotype 2 Cell Wall-Associated Proteins

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Abstract

Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that is distributed throughout the world. Virulence factors and/or markers of the virulent serotype 2 strains have not been fully identified. In this study a simple, rapid, and non-destructive method was used to extract cell wall-associated proteins from SS2 strains. Two virulent strains were compared with one avirulent strain by 2-dimensional electrophoresis (2DE). When the results of the 2DE analyses were combined with the results of mass spectrometry analyses, a total of 40 unique proteins were identified, including 26 antigens (2DE immunoblotting was used as a preliminary study). In addition to a known virulence factor, muramidase-released protein, two new proteins, catabolite control protein A and leucyl aminopeptidase, and nine potential virulence factors were also identified. The formers may be a potential virulence regulator or drug target, and the latter contains plasminogen-binding proteins and molecular chaperones. Our results complemented previous immunoproteomics studies of SS2 strains.

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Abbreviations

SS2:

Streptococcus suis serotype 2

MALDI-TOF-MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

PGM:

Putative phosphoglycerate mutase

TPI:

Triosephosphate isomerase

PGK:

Phosphoglycerate kinase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the “973” program (2006CB504400). We thank Liancheng Lei (Jilin University, China) for providing S. suis strain 606′, and Ailing Zhang and Jinfu Sun for assistance with the experiments.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Jilin University, No.1068, Qinglong Road, Changchun, 130062, People’s Republic of China

    Yingchao Wang, Yuan Dang, Xinglong Wang, Hao Lu, Xiuran Wang, Fuxian Zhang & Linzhu Ren

  2. Institute of Animal Science Academy of Military Medical Sciences, Qinglong Road, Changchun, 130062, People’s Republic of China

    Yingchao Wang, Yuan Dang, Xinglong Wang, Hao Lu, Xiuran Wang, Xulong Lang, Xiaoyan Li, Shuzhang Feng & Fuxian Zhang

  3. Institute of Zoonoses, Jilin University, Qinglong Road, Changchun, 130062, People’s Republic of China

    Xinglong Wang

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  1. Yingchao Wang
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  2. Yuan Dang
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Correspondence to Xinglong Wang.

Additional information

Yingchao Wang, Yuan Dang equally contributed to this work.

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Wang, Y., Dang, Y., Wang, X. et al. Comparative Proteomic Analyses of Streptococcus suis Serotype 2 Cell Wall-Associated Proteins. Curr Microbiol 62, 578–588 (2011). https://doi.org/10.1007/s00284-010-9747-6

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  • DOI: https://doi.org/10.1007/s00284-010-9747-6

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