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Synergism of VAM and Rhizobium on Production and Metabolism of IAA in Roots and Root Nodules of Vigna Mungo

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Abstract

Mature and healthy root nodules of Vigna mungo appeared to contain higher amount of indole-acetic acid (IAA) than non-nodulated roots. Dual effect of VAM fungus, Glomus fasciculatum and the nitrogen-fixing bacteria, Rhizobium sp. on the nodulation of roots of V. mungo was studied. It was recorded that the roots which were inoculated simultaneously with both the symbionts i.e., G. fasciculatum and Rhizobium exhibited greater amount of IAA production than the non-inoculated roots. A tryptophan pool present in the mature nodules and young leaves might serve as a precursor for IAA production in the roots and in the nodules. Activity of IAA-metabolizing enzymes, such as IAA oxidase, peroxidase, and polyphenol oxidase was investigated which indicates the active metabolism of IAA in roots and nodules. The Rhizobium symbiont isolated from fresh nodules of V. mungo produced significant amount of IAA under in vitro condition when tryptophan was added to the medium as precursor. Present study represents some beneficial effects of Rhizobium and G. fasciculatum on the production and metabolism of IAA in roots and nodules of V. mungo. The important physiological implication of the study on IAA production and its metabolism in Rhizobium–Legume–VAM tripartite symbiosis is certainly representing a new approach to satisfy the hormonal balance in the host plant.

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Acknowledgments

The authors are thankful to UGC (SAP-II, Phase-III), Govt. of India for financial assistance.

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Authors and Affiliations

  1. Mycology and Plant Pathology Laboratory, UGC Centre of Advanced Study, Department of Botany, The University of Burdwan, Burdwan, West Bengal, 713104, India

    Jayanta Chakrabarti, Sabyasachi Chatterjee, Sisir Ghosh, Narayan Chandra Chatterjee & Sikha Dutta

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  1. Jayanta Chakrabarti
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  2. Sabyasachi Chatterjee
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  3. Sisir Ghosh
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  4. Narayan Chandra Chatterjee
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  5. Sikha Dutta
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Correspondence to Sikha Dutta.

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Chakrabarti, J., Chatterjee, S., Ghosh, S. et al. Synergism of VAM and Rhizobium on Production and Metabolism of IAA in Roots and Root Nodules of Vigna Mungo . Curr Microbiol 61, 203–209 (2010). https://doi.org/10.1007/s00284-010-9597-2

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