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Fungal Transcript Pattern During the Preinfection Stage (12 h) of Ectomycorrhiza Formed Between Pisolithus tinctorius and Castanea sativa Roots, Identified Using cDNA Microarrays

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Abstract

Transcriptional changes in Pisolithus tinctorius leading to ectomycorrhizal formation in P. tinctorius– Castanea sativa were investigated using a 12-h fungal interaction in vitro system. Using a 3107-cDNA clone microarray, 34 unique expressed sequence tags (ESTs) were found to be differentially expressed. These ESTs represent 14 known genes, 5 upregulated and 9 downregulated, and 20 orphan sequences. Some transcripts of upregulated genes (with unknown function) were previously identified in other mycorrhizal Pisolithus spp. associations. ESTs for S-adenosyl-l-homocysteine hydrolase and several orphan sequences were identified in our system. The identified transcript of downregulated genes involved hydrophobins, 5S, 18S, and 28S ribosomal RNA genes, large subunits of ribosomal RNA (mitochondrial gene), and two types of heat shock proteins. This study demonstrates the high complexity of molecular events involved in the preinfection steps and suggests the utilization of different fungal gene repertories before ectomycorrhizal formation. These data constitute a first contribution for the molecular understanding of early signaling events between P. tinctorius and C. sativa roots during ectomycorrhizal formation.

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Acknowledgments

This work was partially supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/Ministério da Educação–Brazil) through a Ph.D. scholarship and grant conceded to the first author. L. C. Maia acknowledges CNPq (Conselho Nacional de desenvolvimento Científico e Tecnológico, Brazil) support. Dra. Lisete Souza acknowledges FCT (Fundação para a Ciência e Tecnologia, Portugal) support (projects FCT/POCI 2010 and PTDC/MAT/64353/2006). The authors thank Dr. Gregory Mueller (Field Museum of Natural History, Chicago, USA) and Dr. Rita C. C. Maia (CPqAM/FIOCRUZ) for their help in the manuscript review.

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Authors and Affiliations

  1. Laboratório micorrizas, Departamento de Micologia, Universidade Federal de Pernambuco, Recife, Brazil

    Bartolomeu Acioli-Santos & Leonor C. Maia

  2. Unit of Molecular Biology and Plant Biotechnology, Instituto de Ciência Aplicada e Tecnologia, Faculdade de Ciências de Lisboa, Universidade de Lisboa, 1749-016, Lisbon, Portugal

    Mónica Sebastiana, Fernando Pessoa, Andreia Figueiredo, Ana Margarida Fortes, Aladje Baldé & Maria S. Pais

  3. Department of Statistics and Operational Research, Center of Statistics and Applications, Faculdade de Ciências de Lisboa, Universidade de Lisboa, 1749-016, Lisbon, Portugal

    Lisete Sousa

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  1. Bartolomeu Acioli-Santos
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  2. Mónica Sebastiana
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  3. Fernando Pessoa
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  8. Leonor C. Maia
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  9. Maria S. Pais
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Correspondence to Bartolomeu Acioli-Santos.

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Acioli-Santos, B., Sebastiana, M., Pessoa, F. et al. Fungal Transcript Pattern During the Preinfection Stage (12 h) of Ectomycorrhiza Formed Between Pisolithus tinctorius and Castanea sativa Roots, Identified Using cDNA Microarrays. Curr Microbiol 57, 620–625 (2008). https://doi.org/10.1007/s00284-008-9253-2

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  • DOI: https://doi.org/10.1007/s00284-008-9253-2

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