Abstract
Various heterocyclic sulfur compounds such as naphtho[2,1-b]thiophene (NTH) and benzo[b]thiophene (BTH) derivatives can be detected in diesel oil, in addition to dibenzothiophene (DBT) derivatives. Mycobacterium phlei WU-0103 was newly isolated as a bacterial strain capable of growing in a medium with NTH as the sulfur source at 50°C. M. phlei WU-0103 could degrade various heterocyclic sulfur compounds, not only NTH and its derivatives but also DBT, BTH, and their derivatives at 45°C. When M. phlei WU-0103 was cultivated with the heterocyclic sulfur compounds such as NTH, NTH 3,3-dioxide, DBT, BTH, and 4,6-dialkylDBTs as sulfur sources, monohydroxy compounds and sulfone compounds corresponding to starting heterocyclic sulfur compounds were detected by gas chromatography–mass spectrometry analysis, suggesting the sulfur-specific desulfurization pathways for heterocyclic sulfur compounds. Moreover, total sulfur content in 12-fold-diluted crude straight-run light gas oil fraction was reduced from 1000 to 475 ppm S, with 52% reduction, by the biodesulfurization treatment at 45°C with growing cells of M. phlei WU-0103. Gas chromatography analysis with a flame photometric detector revealed that most of the resolvable peaks, such as those corresponding to alkylated derivatives of NTH, DBT, and BTH, disappeared after the biodesulfurization treatment. These results indicated that M. phlei WU-0103 may have a good potential as a biocatalyst for practical biodesulfurization of diesel oil.
Similar content being viewed by others
Literature Cited
JJ Arensdorf AK Loomis PM DiGrazia DJ Monticello PT Pienkos (2002) ArticleTitleChemostat approach for the direct evolution of biodesulfurization gain-of-function mutants Appl Environ Microbiol 68 691–698 Occurrence Handle10.1128/AEM.68.2.691-698.2002 Occurrence Handle1:CAS:528:DC%2BD38XhtV2nsrc%3D Occurrence Handle11823208
SL Borgne R Quintero (2003) ArticleTitleBiotechnological processes for the refining of petroleum Fuel Process Technol 81 155–169
JH Chang SK Rhee YK Chang HN Chang (1998) ArticleTitleDesulfurization of diesel oils by a newly isolated dibenzothiophene-degrading Nocardia sp strain CYKS2 Biotechnol Prog 14 851–855
J Chen Z Ring (2004) ArticleTitleHDS reactivities of dibenzothiophenic compounds in a LC-finer LGO and H2S/NH3 inhibition effect Fuel 83 305–313
WM Coco WE Levinson MJ Crist HJ Hektor A Darzins PT Pienkos CH Squires DJ Monticello (2001) ArticleTitleDNA shuffling method for generating highly recombined genes and evolved enzymes Nat Biotechnol 19 354–359
GA Depauw GF Froment (1997) ArticleTitleMolecular analysis of the sulphur components in a light cycle oil of a catalytic cracking unit by gas chromatography with mass spectrometric and atomic emission detection J Chromatogr A 761 231–247
BR Folsom DR Schieche PM DiGrazia J Werner S Palmer (1999) ArticleTitleMicrobial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-9 Appl Environ Microbiol 65 4967–4972
T Furuya Y Ishii K Noda K Kino K Kirimura (2003) ArticleTitleThermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium phlei WU-F1 FEMS Microbiol Lett 221 137–142
T Furuya K Kirimura K Kino S Usami (2002) ArticleTitleThermophilic biodesulfurization of naphthothiophene and 2-ethylnaphthothiophene by a dibenzothiophene-desulfurizing bacterium, Mycobacterium phlei WU-F1 Appl Microbiol Biotechnol 58 237–240
T Furuya S Takahashi Y Ishii K Kino K Kirimura (2004) ArticleTitleCloning of a gene encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening using indigo production as selective indication Biochem Biophys Res Commun 313 570–575
MJ Grossman MK Lee RC Prince KK Garrett GN George IJ Pickering (1999) ArticleTitleMicrobial desulfurization of crude oil middle-distillate fraction: analysis of the extent of sulfur removal and the effect of removal on remaining sulfur Appl Environ Microbiol 65 181–188 Occurrence Handle1:CAS:528:DyaK1MXjvVygsA%3D%3D Occurrence Handle9872778
K Hirasawa Y Ishii M Kobayashi K Koizumi K Maruhashi (2001) ArticleTitleImprovememt of desulfurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering Biosci Biotechnol Biochem 65 239–246
H Okada N Nomura T Nakahara K Maruhashi (2002) ArticleTitleAnalysis of substrate specificity of the desulfurizing bacterium Mycobacteium sp G3. J Biosci Bioeng 93 228–233
Y Ishii J Konishi H Okada K Hirasawa T Onaka M Suzuki (2000) ArticleTitleOperon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of Paenibacillus sp A11-2. Biochem Biophys Res Commun 270 81–88
K Kirimura T Furuya Y Nishii Y Ishii K Kino S Usami (2000) ArticleTitleBiodesulfurization of dibenzothiophene and its derivatives through selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium Bacillus subtilis WU-S2B J Biosci Bioeng 91 262–266
K Kirimura T Furuya R Sato Y Ishii K Kino S Usami (2002) ArticleTitleBiodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by Rhodococcus sp. strain WU-K2R Appl Environ Microbiol 68 3867–3872
K Kirimura K Harada H Iwasawa T Tanaka Y Iwasaki T Furuya Y Ishii K Kino (2004) ArticleTitleIdentification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria Appl Microbiol Biotechnol 65 703–713
J Konishi Y Ishii T Onaka K Okumura M Suzuki (1997) ArticleTitleThermophilic carbon-sulfur-bond-targeted biodesulfurization Appl Environ Microbiol 63 3164–3169 Occurrence Handle1:CAS:528:DyaK2sXlt1Wisrc%3D
FL Li P Xu CQ Ma LL Luo XS Wang (2003) ArticleTitleDeep desulfurization of hydrodesulfurization-treated diesel oil by a facultative thermophilic bacterium Mycobacterium sp X7B. FEMS Microbiol Lett 223 301–307
S Maghsoudi M Vossoughi A Kheirolomoon E Tanaka S Katoh (2001) ArticleTitleBiodesulfurization of hydrocarbons and diesel fuels by Rhodococcus sp strain P32C1 Biochem Eng J 8 151–156
DJ Monticello (2000) ArticleTitleBiodesulfurization and the upgrading of petroleum distillates Curr Opin Biotechnol 11 540–546
SG Mossner MJL Alda LC Sander ML Lee SA Wise (1999) ArticleTitleGas chromatographic retention behavior of polycyclic aromatic sulfur heterocyclic compounds, (dibenzothiophene, naphtho[b]thiophenes, benzo[b]thiophenes and alkyl-substituted derivatives) on stationary phases of different selectivity J Chromatogr A 841 207–228
T Ohshiro Y Izumi (1999) ArticleTitleMicrobial desulfurization of organic sulfur compounds in petroleum Biosci Biotechnol Biochem 63 1–9
SK Rhee JH Chang YK Chang HN Chang (1998) ArticleTitleDesulfurization of dibenzothiophene and diesel oils by a newly isolated Gordona strain, CYKS1 Appl Environ Microbiol 64 2327–2331 Occurrence Handle1:CAS:528:DyaK1cXjslWhtLc%3D Occurrence Handle9603863
Acknowledgments
This study was supported in part by Industrial Technology Research Grant Program in ‘01B63002c (to Y. Ishii) from New Energy and Industrial Technology Development Organization (NEDO) subsidized by the Ministry of Economy, Trade and Industry of Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ishii, Y., Kozaki, S., Furuya, T. et al. Thermophilic Biodesulfurization of Various Heterocyclic Sulfur Compounds and Crude Straight-Run Light Gas Oil Fraction by a Newly Isolated Strain Mycobacterium phlei WU-0103. Curr Microbiol 50, 63–70 (2005). https://doi.org/10.1007/s00284-004-4403-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-004-4403-7