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A common F13A1 intron 1 variant IVS1+12(A) is associated with mild FXIII deficiency in Caucasian population

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Abstract

Mild factor XIII deficiency is an underdiagnosed coagulation disorder. Considering the large number of coding and non-coding polymorphisms identified in the F13A1 gene, there is a possibility that some of these might result in alterations of plasma FXIII levels and cause mild FXIII deficiency. Recently, a homozygous F13A1 gene intron 1 variant (IVS1+12C>A) was found in a patient with FXIII deficiency. In vitro expression studies for this variant demonstrated its lowering effect on FXIII levels. In order to determine the impact of this variant on a population level, we analysed the prevalence of this variant in three clinically and genetically defined population cohorts: an apparently healthy control cohort C1 (n = 102), a mild FXIII deficiency cohort C2 with no detectable F13A1 or F13B gene mutations (n = 183) and a mild FXIII-A deficiency cohort C3 exhibiting heterozygous F13A1 mutations (n = 37). FXIII activity was determined using photometric assay on plasma samples. The F13A1 gene intron 1 variant was analysed by direct sequencing. The C1 cohort showed a normal distribution of FXIII activity (mean 114.1 ± 20.86 %). Mean FXIII activity levels for the C2 and C3 cohorts were 54.45 ± 11.12 % and 44.21 ± 10.16 %, respectively. The frequencies of minor allele (A) were 0.07 in C1 cohort, 0.19 in C2 cohort and 0.11 in C3 cohort. The difference in minor allele frequencies for the C1 and C2 cohorts were highly significant (p < 0.001). The greater frequency of the IVS1+12(A) variant among C2 cohort patients suggests that this polymorphism is associated with mild FXIII deficiency.

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Ethical standard

Informed consent was obtained from all patients for being included in the study.

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The authors have no competing interests.

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Authors and Affiliations

  1. Institute of Experimental Haematology and Transfusion Medicine, University Clinic Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany

    Vytautas Ivaskevicius, Arijit Biswas, Anne Thomas, Sophie Lyonga, Ursula Harbrecht, Hans-Joerg Hertfelder, Natascha Marquardt & Johannes Oldenburg

  2. Gerinnungszentrum Rhein-Ruhr, Duisburg, Germany

    Hannelore Rott, Susan Halimeh & Guenther Kappert

  3. Vivantes Clinic in Friedrichshain, Berlin, Germany

    Robert Klammroth

  4. Zentrum für Blutgerinnungsstörungen, Leipzig, Germany

    Ute Scholz

  5. Klinikum Braunschweig, Braunschweig, Germany

    Wolfgang Eberl

  6. MVZ für Hämostaseologie, Laboratoriums, Transfusions- und Allgemeinmedizin Bonn, Bonn, Germany

    Christine Gnida

Authors
  1. Vytautas Ivaskevicius
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  2. Arijit Biswas
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  3. Anne Thomas
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  4. Sophie Lyonga
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  5. Hannelore Rott
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  6. Susan Halimeh
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  7. Guenther Kappert
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  8. Robert Klammroth
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  9. Ute Scholz
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  10. Wolfgang Eberl
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  11. Ursula Harbrecht
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  12. Christine Gnida
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  13. Hans-Joerg Hertfelder
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  14. Natascha Marquardt
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  15. Johannes Oldenburg
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Corresponding authors

Correspondence to Vytautas Ivaskevicius or Arijit Biswas.

Additional information

Vytautas Ivaskevicius and Arijit Biswas are first authors and equally contributed to this study.

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Ivaskevicius, V., Biswas, A., Thomas, A. et al. A common F13A1 intron 1 variant IVS1+12(A) is associated with mild FXIII deficiency in Caucasian population. Ann Hematol 92, 975–979 (2013). https://doi.org/10.1007/s00277-013-1724-2

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  • DOI: https://doi.org/10.1007/s00277-013-1724-2

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