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In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy

  • Original Article
  • Basic Science/Experimental
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Abstract

Objective

In this study, we investigate the biological characteristics of ADSCs from the liposuction area in patients with hemifacial atrophy in vitro.

Methods

ADSCs were respectively extracted from the donor site of patients with hemifacial atrophy and healthy ones. ADSCs of two groups were respectively tested for proliferation ability, phenotype, multipotency, migration ability, self-repair ability, apoptosis, and autophagy. Exosomes extracted from the supernatant of two groups were detected by NTA particle size, electron microscopy (TEM), and WB for CD63 and TSG10, respectively.

Results

CCK-8 showed a statistically less increase in cell proliferation in PHA-ADSCs after the sixth day. ADSCs in both groups had typical phenotypes and multidirectional abilities. PHA-ADSCs exhibited weaker droplet formation. The cell migration ability in PHA-ADSCs was weaker tested by Transwell assay. The live/dead proportion calculated by ImageJ following calcein-AM/PI double staining revealed live cells in PHA-ADSCs was 46.11% compared with 54.21% in NORM-ADSCs after OGD treatment. A significant down-regulation of ATG7 and ATG12 and a higher percentage of apoptosis were found in PHA-ADSCs. A significant up-regulation of BAX occurred in PHA-ADSCs.ARPC5 expression in the PHA group was extremely distinct down-regulated.CDKN1A and CDKN2A expression in the PHA group was significantly up-regulated.WB analyses confirmed that both groups' ADSCs-Exosomes surface markers CD63 and TSG101 were positively expressed but varied significantly.

Conclusions

PHA-ADSCs exhibited a poorer proliferation ability, higher apoptosis percentage, weaker lipid droplets formation, weaker cell migration, poorer intolerance to OGD, aging earlier, and weaker self-renewal and repairability.PHA-ADSCs-Exosomes showed low expressions of CD63 and TSG101.This study provides strong evidence that the addition of exosomes with specific cytokines can improve the fat survival rate after fat filling in patients with hemifacial atrophy.

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Data Availability

All data generated or analyzed during this study are included in this article. Further enquiries can be directed to the corresponding author.

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Funding

The authors received National Multi-disciplinary Collaborative Diagnosis and Treatment capacity Building project for Critical Diseases (No.19038) and CAMS-Innovation Fund for Medical Sciences (No.2020-12M-C&T-A-018) support for the research, authorship, and publication of this article.

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Authors and Affiliations

  1. Plastic Surgery Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Qiuni Gao, Zuoliang Qi, Xiaolei Jin, Zhenyu Yang, Xihang Yuan, Yuling Hu & Xiaonan Yang

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  1. Qiuni Gao
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  2. Zuoliang Qi
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  3. Xiaolei Jin
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  4. Zhenyu Yang
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Contributions

QG, ZY, YH, XY, XY, XJ and ZQ performed the research.QG,XJ and ZQ designed this study. QG and ZY analyzed the data. QG, ZY, XY, YH and XY followed up with the patients and wrote this paper. XJ and ZQ provided advice and critical discussions on the project. All of the authors read and approved the final version of this paper and participated in reviewing and editing the manuscript and approved the final version.

Corresponding authors

Correspondence to Zuoliang Qi or Xiaolei Jin.

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The authors declare that they have no conflict of interest to disclose.

Ethical Approval

Written informed consent was obtained from two patients for their portrait rights in accordance with the institutional ethical committee of Plastic Surgery Hospital (Institute), Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College, and the approval number was 2021-203.And this article did not contain any studies with human participants or animals performed by any of the authors.

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Gao, Q., Qi, Z., Jin, X. et al. In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy. Aesth Plast Surg 46, 2526–2538 (2022). https://doi.org/10.1007/s00266-022-02902-x

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