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Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

  • Original Article
  • Basic Science/Experimental
  • Published:
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Abstract

Background

Autologous fat grafting is a common procedure performed for cosmetic and reconstructive purposes. Unpredictable graft survival is a major drawback, and a variety of improvements on technique such as using antioxidants have been reported to increase retention. The authors examined whether a natural rosemary plant extract known with antioxidant properties, rosmarinic acid, could improve the survival of the adipose tissue.

Methods

Inguinal fat pads were harvested from 24 Sprague Dawley rats and implanted to the parascapular area. Rats were divided into three groups: rosmarinic acid and ethanol as solvent (RA), ethanol (E), and serum physiologic (C). These substances were administered intraperitoneally daily for 1 week and once a week for 7 weeks. Volume and weight measurements, blood specimens, weight and volume records, and histopathologic examinations were performed and analyzed.

Results

The RA group demonstrated lower TOS, TGF-beta, TNF-alpha, and MDA values compared to E and C groups. TGF-beta increase was statistically insignificant, but TOS, TNF-alpha, MDA decrease was found statistically significant. Weight and volume losses were lower in the RA and E groups compared to the C group. The difference between the RA and E groups in terms of weight and volume loss was statistically insignificant. Histopathologically fat necrosis, inflammation, and fibrosis were less in the RA group compared to the E and C groups (p < 0.05).

Conclusion

Rosmarinic acid increased the fat graft volume retained and decreased cyst formation and abscess formation in the rat model. Further studies can be undertaken to investigate rosmarinic acid’s local application as a tumescent and safety in humans.

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Funding

All of the expenses of this study have been funded by the corresponding author.

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Authors and Affiliations

  1. Department of Plastic, Reconstructive, and Aesthetic Surgery, Haydarpasa Numune Research and Training Hospital, Istanbul, Turkey

    Baris Cin, Nesibe Sinem Ciloglu & Shahruk Omar

  2. Department of Pathology, Sultan Abdulhamit Han Research and Training Hospital, Istanbul, Turkey

    Neslihan Kaya Terzi

  3. Edirne Sultan 1. Murat Devlet Hastanesi Plastik, Rekonstrüktif ve Estetik Cerrahi Kliniği, Hadmağa Mevkii, Helvacısakaoğlu Sokak, 22030, Edirne Merkez, Edirne, Turkey

    Baris Cin

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  1. Baris Cin
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  2. Nesibe Sinem Ciloglu
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Correspondence to Baris Cin.

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All of the authors declare that they have no conflict of interest.

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This study was approved by the ethical review board of Acibadem University. All applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Cin, B., Ciloglu, N.S., Omar, S. et al. Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model. Aesth Plast Surg 44, 177–185 (2020). https://doi.org/10.1007/s00266-019-01519-x

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  • DOI: https://doi.org/10.1007/s00266-019-01519-x

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