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Inhibition of Oxygen-Glucose Deprivation-Induced Apoptosis of Human Adipose-Derived Stem Cells by Genetic Modification with Antiapoptotic Protein Bcl-2

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Abstract

Background

Adipose-derived stem cells (ADSCs) have become a promising tool for a wide range of cell-based therapies. However, transplanted ADSCs do not survive well under ischemic conditions. In this study we aimed to inhibit oxygen-glucose deprivation (OGD)-induced apoptosis of human ADSCs by genetic modification with antiapoptotic protein Bcl-2.

Methods

After isolation and culture, the phenotypes of human ADSCs at passage 3 were analyzed by flow cytometry. Then, genetic modification of ADSCs with Bcl-2 was carried out. Bcl-2 gene transfection was verified by Western blot analysis and multipotent differentiation properties were evaluated in Bcl-2-modified ADSCs (Bcl-2-ADSCs). Apoptosis was evaluated by a TUNEL assay under ischemic conditions induced by OGD. Apoptotic nuclei were also assessed and quantified by Hoechst staining.

Results

The cultured ADSCs expressed stem cell-associated markers CD29, CD34, CD44, and CD90, but not fibroblast marker HLA-DR or hematopoietic stem cell marker CD133. The Bcl-2 gene was transferred into ADSCs efficiently, and Bcl-2-ADSCs differentiated into adipocytes, chondrocytes, and osteoblasts. In addition, Bcl-2 overexpression reduced the percentage of apoptotic Bcl-2-ADSCs by 38 % under OGD.

Conclusion

Our results indicate that Bcl-2 overexpression through gene transfection inhibits apoptosis of ADSCs under ischemic conditions.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (81272108), the Key Talent Foundation of China (RC2007009), and the 333 Project Talent Foundation of China (NJGL2007116).

Conflicts of interest

The authors have no conflicts of interest or financial ties to declare.

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Authors and Affiliations

  1. Department of Burns and Plastic Surgery, The Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, 210008, China

    Ziwei Cui, Liangyun Shen & Qian Tan

  2. Department of Burns and Plastic Surgery, Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, 210008, China

    Yue Lin, Shuqin Wang, Dongfeng Zheng & Qian Tan

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  1. Ziwei Cui
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Correspondence to Qian Tan.

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Cui, Z., Shen, L., Lin, Y. et al. Inhibition of Oxygen-Glucose Deprivation-Induced Apoptosis of Human Adipose-Derived Stem Cells by Genetic Modification with Antiapoptotic Protein Bcl-2. Aesth Plast Surg 38, 779–787 (2014). https://doi.org/10.1007/s00266-014-0354-y

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