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Cadaveric allograft microbiology

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Abstract

This study aims to determine the contamination rate of cadaveric bone allograft and blood cultures retrieved from 119 donors within Leicester between 1990 and 2003. A contamination rate of 27% was present, with 120 of 437 bone allografts culturing positive at the time of retrieval. Similarly, a contamination rate of 37% was present, with 40 of 107 blood samples culturing positive. The time interval between death and procurement did not influence blood contamination. Coagulase-negative Staphylococcus was the commonest organism isolated in both blood and bone cultures. One donor had Clostridium grown in their blood culture. The available evidence confirms similar contamination rates with other studies. The majority of organisms isolated were skin commensals with a low rate of contamination of highly pathogenic organisms such as Clostridium.

Résumé

Cette étude a pour but de déterminer le taux de contamination des allogreffes osseuses cadavériques et des hémocultures chez 119 donneurs, entre 1990 et 2003, à Leicester. Un taux de contamination de 27% était présent avec 120 des 437 allogreffes avec une culture positive au moment du prélèvement. De la même façon, un taux de contamination de 37% était présent avec 40 des 107 prélèvements de sang avec une culture le positive. L’intervalle de temps entre la mort et l’acquisition du prélèvement n’a pas influencé la contamination du sang. Le staphylocoque coagulase négative était l’organisme plus fréquent isolé dans le sang et dans l’os. Un donneur avait un Clostridium dans l’hémoculture. Les résultats confirment des taux de contamination voisins de ceux des autres études. La majorité des organismes isolés sont des commensaux de la peau avec un bas taux de contamination d’organismes hautement pathogènes tels que Clostridium.

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References

  1. Bettin D, Harms C, Polster J, Niemeyer T (1998) High incidence of pathogenic microorganisms in bone allografts explanted in the morgue. Acta Orthop Scand 69:311–314

    CAS  PubMed  Google Scholar 

  2. British Association of Tissue Banking (1999) General standards for tissue banking. BATB

  3. Centre for Disease Control and Prevention (2001) Notice to readers: unexplained deaths following knee surgery. MMWR Morb Mortal Wkly Rep 50:1080

    PubMed  Google Scholar 

  4. Deijkers RLM, Bloem RM, Petit PLC, Brand R, Vehmeyer SBW, Veen MR (1997) Contamination of bone allografts: analysis of incidence and predisposing factors. J Bone Joint Surg Br 79:161–166

    Article  CAS  PubMed  Google Scholar 

  5. Dolan CT, Brown AL, Ritts RE (1971) Microbiological examination of post-mortem tissues. Arch Pathol 92:206

    CAS  PubMed  Google Scholar 

  6. European Association of Musculo Skeletal Transplantation (1994) Standards for tissue banking and current developments. EAMST

  7. Ivory JP, Thomas IH (1993) Audit of a bone bank. J Bone Joint Surg Br 75:355–357

    CAS  PubMed  Google Scholar 

  8. Journeaux SF, Johnson N, Bryce SL, Friedman SJ, Sommerville SMM, Morgan DAF (1999) Bacterial contamination rates during bone allograft retrieval. J Arthroplasty 14:677–681

    Article  CAS  PubMed  Google Scholar 

  9. Kumta SM, Kendal N, Lee YL, Panozzo A, Leung PC, Chow TC (1997) Bacterial colonization of bone allografts related to increased interval between death and procurement: an experimental study in rats. Arch Orthop Trauma Surg 116:496–497

    Article  CAS  PubMed  Google Scholar 

  10. Lord CF, Gebhardt MC, Tomford WW, Mankin HJ (1988) Infection in bone allografts: incidence, nature, and treatment. J Bone Joint Surg Am 70:369–376

    CAS  PubMed  Google Scholar 

  11. Malinin TI, Martinez OV, Brown MD (1985) Banking of massive osteoarticular and intercalary bone allografts: 12 years’ experience. Clin Orthop 197:44–57

    PubMed  Google Scholar 

  12. Malinin TI, Buck BE, Temple HT, Martinez OV, Fox WP (2003) Incidence of clostridial contamination in donors’ musculoskeletal tissue. J Bone Joint Surg Br 85:1051–1054

    Article  CAS  PubMed  Google Scholar 

  13. Martinez OV, Malinin TI, Valla PH, Flores A (1985) Postmortem bacteriology of cadaver tissue donors: an evaluation of blood cultures as an index of tissue sterility. Diagn Microbiol Infect Dis 3:193–200

    Article  CAS  PubMed  Google Scholar 

  14. Sutherland AG, Raafat A, Yates P, Hutchison JD (1997) Infection associated with the use of allograft bone from the north east Scotland bone bank. J Hosp Infect 35:215–222

    Article  CAS  PubMed  Google Scholar 

  15. Tomford WW, Thongphasuk J, Mankin HJ, Ferraro MJ (1990) Frozen musculoskeletal allografts: a study of the clinical incidence and causes of infection associated with their use. J Bone Joint Surg Am 72:1137–1143

    CAS  PubMed  Google Scholar 

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Correspondence to T. Ibrahim.

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Ibrahim, T., Stafford, H., Esler, C.N.A. et al. Cadaveric allograft microbiology. International Orthopaedics (SICOT) 28, 315–318 (2004). https://doi.org/10.1007/s00264-004-0579-5

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  • DOI: https://doi.org/10.1007/s00264-004-0579-5

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