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Degradation of 2,4,5-trichlorophenol and 2,3,5,6-tetrachlorophenol by combining pulse electric discharge with bioremediation

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Degradation of 2,4,5-trichlorophenol (2,4,5-TCP) and 2,3,5,6-tetrachlorophenol (TeCP) was studied using a two-stage approach that utilized efficient pulse electric discharge (PED) followed by biological degradation with a consortium from acclimated return activated sludge. The chlorinated phenols were treated in the PED reactor as an aerosol at a voltage of 55–60 kV, a frequency of 385 Hz, a current of 50–60, and with a 200-ns pulse. As determined by gas chromatography and mass spectrometry (GC/MS), the first stage converted 500 ppm 2,4,5-TCP to 163 ppm 2,4,5-TCP and dimethyldecene, dichloronaphthalenol, octyl acetate, and silyl esters. The total carbon content of 2,4,5-TCP after PED treatment was determined to be 228 ± 35 ppm. The remaining 2,4,5-TCP and the products formed were then mineralized by the acclimated activated sludge in shake flasks; the initial rate of degradation of 2,4,5-TCP was calculated to be 5 nmol min−1 mg protein−1 at 163 ppm initial concentration (three orders of magnitude higher than the only rate found in the literature). By combining the two techniques, a synergistic effect (2.3-fold increase in the concentration of 2,4,5-TCP degraded and 3.3-fold increase in total carbon degraded) was observed, in that bacteria without any treatment degraded a maximum of 70 ppm 2,4,5-TCP but after PED treatment 163 ppm 2,4,5-TCP was degraded. TeCP was also mineralized by the acclimated activated sludge after treatment with PED. This two-stage approach was also evaluated using a continuous 1-l fluidized-bed reactor.

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Received: 3 November 1998 / Received revision: 28 February 1999 / Accepted: 14 March 1999

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Chauhan, S., Yankelevich, E., Bystritskii, V. et al. Degradation of 2,4,5-trichlorophenol and 2,3,5,6-tetrachlorophenol by combining pulse electric discharge with bioremediation. Appl Microbiol Biotechnol 52, 261–266 (1999). https://doi.org/10.1007/s002530051519

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  • DOI: https://doi.org/10.1007/s002530051519

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