Abstract
In this work the effects of individual purified cellulases of Trichoderma reesei were studied in the enzyme-aided bleaching of kraft pulps. The cellobiohydrolases I and II, when used alone, had no positive effect on the bleachability of kraft pulps. The endoglucanase I (EG I), however, acted on pulp similarly to xylanases and with an enzyme dosage of 0.1 mg/g a clear increase in pulp brightness could be observed. Due to the unspecificity of this enzyme, the viscosity of the pulp was simultaneously decreased. Of the cellulases, EG II was clearly most detrimental in reducing the pulp viscosity. Hence, the action of purified cellulases of T. reesei on pulp as a substrate differs profoundly, and all cellulases are not detrimental to the pulp properties.
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References
Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing for assay of xylanase activity. J Biotechnol 23:257–270
Bailey M, Siika-aho M, Valkeajärvi A, Penttilä M (1993) Hydrolytic properties of two cellulases of Trichoderma reesei expressed in yeast. Biotechnol Appl Biochem 17:65–76
Buchert J, Ranua M, Kantelinen A, Viikari L (1992) The role of two Trichoderma reesei xylanases in bleaching of pine kraft pulp. Appl Microbiol Biotechnol 37:825–829
Buchert J, Salminen J, Siika-aho M, Ranua M, Viikari (1993a) The role of Trichoderma reesei xylanase and mannanase in the treatment of softwood kraft pulp prior to bleaching. Holzforschung 7:473–478
Buchert J, Siika-aho M, Bailey M, Puls J, Valkeajärvi A, Pere J, Viikari L (1993b) Quantitative determination of wood-derived soluble oligosaccharides. Biotechnol Tech (in press)
Enari T-M, Niku-Paavola M-L (1987) Enzymatic hydrolysis of cellulose. CRC Crit Rev Biotechnol 5:67–87
Hortling B, Korhonen M, Buchert J, Sundquist J, Viikari L (1994) The leachability of lignin from kraft pulps after xylanase treatment. Holzforschung (in press)
IUPAC (International Union of Pure and Applied Chemistry) (1989) Measurement of cellulase activities. Pure Appl Chem 59:257–268
Kantelinen A, Sundquist J, Linko M, Viikari L (1991) The role of reprecipitated xylan in the enzymatic bleaching of kraft pulp. In: Proceedings of the 6th International Symposium on Wood Pulp Chemistry, Melbourne 1991, vol. I, pp 493–500
Kantelinen A, Hortling B, Sundquist J, Linko M, Viikari L (1993) Proposed mechanism of the enzymatic bleaching of kraft pulp with xylanases. Holzforschung 47:318–324
Koponen R (1991) Enzyme systems prove their potential. Pulp Paper Int 33:20–25
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Niku-Paavola M-L, Lappalainen A, Enari T-M, Nummi M (1985) A new appraisal of the endoglucanases of the fungus Trichoderma reesei. Biochem J 231:75–81
Niku-Paavola M-L, Enari T-M, Nummi M (1986) Trichoderma reesei cellobiohydrolase II. Biotechnol Appl Biochem 8:449–458
Paice M, Bernier M, Jurasek L (1988) Viscosity enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene. Biotechnol Bioeng 32:235–239
Shoemaker S, Watt K, Tsitovsky G, Cox R (1983) Properties and characterization of cellulases purified from Trichoderma reesei strain L27. Bio/Technology 1:687–690
Stone JE, Scallan AM (1968) The effect of component removal upon the porous structure of a cell wall of wood. Pulp Paper Mag Can 69:69–74
Stone JE, Scallan AM, Donefer E, Ahlgren E (1969) Digestibility as a simple function of a molecule of a similar size to a cellulase enzyme. Adv Chem Ser 95:219–241
Ståhlbrand H, Siika-aho M, Tenkanen M, Viikari L (1993) Purification and characterization of two ß-mannanases from Trichoderma reesei. J Biotechnol 29:229–242
Suominen P, Mäntylä A, Saarelainen R, Paloheimo M, Fagerström R, Parkkinen E, Nevalainen H (1992) Genetic engineering of Trichoderma reesei to produce suitable enzyme combinations for applications in the pulp and paper industry. In: Kuwahara M, Shimada M (eds) Biotechnology in the pulp and paper industry. UNI, Tokyo, pp 439–445
Tenkanen M, Puls J, Poutanen K (1992) Two major xylanases of Trichoderma reesei. Enzyme Microb Technol 14:566–574
Viikari L, Sundquist J, Kettunen J (1991) Xylanase enzymes promote pulp bleaching. Paper Timber 73:384–389
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Buchert, J., Ranua, M., Siika-aho, M. et al. Trichoderma reesei cellulases in the bleaching of kraft pulp. Appl Microbiol Biotechnol 40, 941–945 (1994). https://doi.org/10.1007/BF00174003
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DOI: https://doi.org/10.1007/BF00174003