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Engineering Clostridium for improved solvent production: recent progress and perspective

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Abstract

Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.

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Acknowledgments

This material is based upon the work supported by the Department of Energy’s Office of Energy Efficiency and Renewable Energy (EERE) under the Bioenergy Technologies Office, Award Number DE-EE0007005.

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This study was funded by the Department of Energy (Grant number: DE-EE0007005).

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  1. School of Bioengineering, Dalian University of Technology, Dalian, 116024, China

    Chi Cheng

  2. Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH, 43210, USA

    Chi Cheng, Teng Bao & Shang-Tian Yang

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Cheng, C., Bao, T. & Yang, ST. Engineering Clostridium for improved solvent production: recent progress and perspective. Appl Microbiol Biotechnol 103, 5549–5566 (2019). https://doi.org/10.1007/s00253-019-09916-7

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