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Effect of methyl-β-cyclodextrin on gene expression in microbial conversion of phytosterol

  • Genomics, transcriptomics, proteomics
  • Published:
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Abstract

Modified β-cyclodextrins are widely used for the enhancement of microbial conversions of lipophilic compounds such as steroids. Multiple mechanisms of cyclodextrin-mediated enhancement of phytosterol bioconversion by mycobacteria had previously been shown to include steroid solubilization, alterations in the cell wall permeability for both steroids and nutrients, facilitation of protein leaking, and activity suppression of some steroid-transforming enzymes.In this work, we studied whether cyclodextrins might affect expression of the genes involved in the steroid catabolic pathway. Phytosterol bioconversion with 9α-hydroxy-androst-4-ene-3,17-dione accumulation by Mycobacterium sp. VKM Ac-1817D in the presence of methylated β-cyclodextrin (MCD) was investigated. RNA sequencing of the whole transcriptomes in different combinations of phytosterol and MCD showed a similar expression level of the steroid catabolism genes related to the KstR-regulon and was responsible for side chain and initial steps of steroid core oxidation; whereas, induction levels of the genes related to the KstR2-regulon were attenuated in the presence of MCD in this strain. The data were attenuated with quantitative real-time PCR.The results contribute to the understanding of cyclodextrin effects on microbial steroid conversion and provide a basis for the use of cyclodextrins as expression enhancers for studies of sterol catabolism in actinobacteria.

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Acknowledgements

This work was supported by a Russian Science Foundation (Grant No. 14-24-00169).

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Authors and Affiliations

  1. A.N. Belozersky Research Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie gory, h. 1, b. 40, Moscow, Russian Federation, 119991

    Victoria Y. Shtratnikova

  2. Russian Academy of Sciences, Institute for Information Transmission Problems, Bolshoy Karetny per., h. 19, b. 1, Moscow, Russian Federation, 127051

    Mikhail I. Schelkunov

  3. Russian Academy of Sciences, G.K. Skryabin Institute of Biochemistry & Physiology of Microorganisms, Prospekt Nauki, h. 5, Puschino, Moscow region, Russian Federation, 142290

    Dmitry V. Dovbnya, Eugeny Y. Bragin & Marina V. Donova

  4. People’s Friendship University of Russia, Miklukho-Maklaya str. 6, Moscow, Russian Federation, 117198

    Marina V. Donova

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  1. Victoria Y. Shtratnikova
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  2. Mikhail I. Schelkunov
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  3. Dmitry V. Dovbnya
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  4. Eugeny Y. Bragin
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  5. Marina V. Donova
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Contributions

VS and EB designed and carried out mRNA isolation, high-throughput sequencing, RT-PCR experiments, and data analysis; MS made bioinformatics calculations; DD designed and carried out growth, bioconversion, and induction experiments; VS, MS, DD, and MD wrote the manuscript; MD coordinated the project, participated in the conception, drafting, and revision of the manuscript. All authors read and approved the final manuscript before submission.

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Correspondence to Victoria Y. Shtratnikova.

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This work was supported by a Russian Science Foundation (Grant No. 14-24-00169).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Shtratnikova, V.Y., Schelkunov, M.I., Dovbnya, D.V. et al. Effect of methyl-β-cyclodextrin on gene expression in microbial conversion of phytosterol. Appl Microbiol Biotechnol 101, 4659–4667 (2017). https://doi.org/10.1007/s00253-017-8288-3

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  • DOI: https://doi.org/10.1007/s00253-017-8288-3

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