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Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems

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Abstract

During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described—mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymerase depends on the application itself. This review gives an overview of the most commonly used DNA polymerases used for PCR application: KOD, Pab (Isis™), Pfu, Pst (Deep Vent™), Pwo, Taq, Tbr, Tca, Tfi, Tfl, Tfu, Tgo, Tli (Vent™), Tma (UITma™), Tne, Tth and others.

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The author thanks Prof. A. Steinbüchel for his support this review.

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    Kay Terpe

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Terpe, K. Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 97, 10243–10254 (2013). https://doi.org/10.1007/s00253-013-5290-2

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  • DOI: https://doi.org/10.1007/s00253-013-5290-2

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