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Binding characteristics of a bacterial expansin (BsEXLX1) for various types of pretreated lignocellulose

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Abstract

BsEXLX1 from Bacillus subtilis is the first discovered bacterial expansin as a structural homolog of a plant expansin, and it exhibited synergism with cellulase on the cellulose hydrolysis in a previous study. In this study, binding characteristics of BsEXLX1 were investigated using pretreated and untreated Miscanthus x giganteus in comparison with those of CtCBD3, a cellulose-binding domain from Clostridium thermocellum. The amounts of BsEXLX1 bound to cellulose-rich substrates were significantly lower than those of CtCBD3. However, the amounts of BsEXLX1 bound to lignin-rich substrates were much higher than those of CtCBD3. A binding competition assay between BsEXLX1 and CtCBD3 revealed that binding of BsEXLX1 to alkali lignin was not affected by the presence of CtCBD3. This preferential binding of BsEXLX1 to lignin could be related to root colonization in plants by bacteria, and the bacterial expansin could be used as a lignin blocker in the enzymatic hydrolysis of lignocellulose.

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Acknowledgments

This work was supported by grants from the Advanced Biomass R&D Center of Korea (2011-0031353) and the Intelligent Synthetic Biology Center (No. 2011-0031953) funded by the Korean Government (MEST). K.H.K acknowledges financial support from the Energy Biosciences Institute (EBI), Berkeley, CA, USA and I.J.K. is grateful to the instrumental training and technical support provided by Drs. Mara Bryan and Stefan Bauer at the EBI. Facility support at Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety is also acknowledged.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea

    In Jung Kim, Hyeok-Jin Ko, In-Geol Choi & Kyoung Heon Kim

  2. Energy Biosciences Institute, Berkeley, CA, 94720, USA

    Tae-Wan Kim

  3. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14850, USA

    Ki Hyun Nam

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  1. In Jung Kim
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  2. Hyeok-Jin Ko
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Correspondence to Kyoung Heon Kim.

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Kim, I.J., Ko, HJ., Kim, TW. et al. Binding characteristics of a bacterial expansin (BsEXLX1) for various types of pretreated lignocellulose. Appl Microbiol Biotechnol 97, 5381–5388 (2013). https://doi.org/10.1007/s00253-012-4412-6

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