Abstract
Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on morphology, physiological tests (API 20 C AUX), and 5.8S-ITS sequence, the isolate was identified as Candida krusei. Kinetic characterization demonstrated that carbonyl reductase from C. krusei ZJB-09162 preferred NADH to NADPH as cofactor, indicating it might be a new carbonyl reductase. (R)-1,3-Butanediol was produced in 19.8 g/L, 96.6% conversion, and 99.0% ee at optimal pH 8.5, 35 °C with a 2:1 molar ratio of glucose to 4H2B. In order to achieve higher product titer, the substrate loading was optimized in fixed catalysts and fixed substrate/catalysts ratio mode. The bioreduction of 4-hydroxy-2-butanone at a concentration of 45.0 g/L gave (R)-1,3-butanediol in 38.7 g/L and 83.9% conversion. Therefore, C. krusei ZJB-09162 was, for the first time, proven to be a promising biocatalyst for enzymatic preparation of (R)-1,3-butanediol.
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Acknowledgements
We are grateful to the Key Scientific and Technological Programs of Zhejiang Province (no. 2009 C13033-3), 973 Program (no. 2011CB710806), Educational Commission of Zhejiang Province of China (no. Y201018390), and the Key Innovation Research Group Programs of Zhejiang Province (no. 2011R09043-04) for the financial support of this research.
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Zheng, RC., Ge, Z., Qiu, ZK. et al. Asymmetric synthesis of (R)-1,3-butanediol from 4-hydroxy-2-butanone by a newly isolated strain Candida krusei ZJB-09162. Appl Microbiol Biotechnol 94, 969–976 (2012). https://doi.org/10.1007/s00253-012-3942-2
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DOI: https://doi.org/10.1007/s00253-012-3942-2