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Highly sensitive field test lateral flow immunodiagnostics of PVX infection

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An Erratum to this article was published on 28 October 2011

Abstract

A test system is described and expanded upon for mass field immunochromatography assay on porous membrane carriers for rapid diagnostics of potato virus X (PVX) in potato leaf tissue and sprout extracts using colloidal gold nanoparticles as a marker. Sensitivity of the assay developed for PVX identification is found to be comparable to the sensitivity of solid-phase sandwich-ELISA. Complete assay time does not exceed 15 min, and the lower limit of the PVX detection in non-clarified leaf extract is 2 ng/ml. A single measurement requires 0.1–0.2 ml (3–5 drops) of tested solution only (extracted from 10–20 mg of potato leaf tissue or sprouts). The simplicity and reliability of the method makes it especially efficient in direct rapid monitoring of many infected potato specimens in the field, as verified by field trials of 360 clones of 28 domestic and foreign cultivars of potato. A diagnostic kit for routine analyses of potato viral infections both in the laboratory and in the field is described and expanded upon.

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Acknowledgments

This work was supported in part by Russian Foundation for Basic Research, grant 08-04-13613.

We deeply appreciate Dr. Emma Hoyle for English language assistance.

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Authors and Affiliations

  1. A.N. Belozersky Institute of Physico-chemical Biology, Moscow State University, Vorob’evy gory 1, bld. 40, 119991, Moscow, Russia

    Yuri F. Drygin & Joseph G. Atabekov

  2. Department of Biology, Moscow State University, Vorob’evy gory 1, bld. 12, 119991, Moscow, Russia

    Anatoly N. Blintsov & Joseph G. Atabekov

  3. Department of Chemistry, Moscow State University, Vorob’evy gory 1, bld. 11, 119991, Moscow, Russia

    Vitaly G. Grigorenko & Alexander P. Osipov

  4. ZAO “NVO Immunotek”, Vorob’evy gory 1, bld. 11a, 119991, Moscow, Russia

    Irina P. Andreeva

  5. All-Russian Potato Research Institute, 140051, Moscow, Russia

    Yuri A. Varitzev, Alexander I. Uskov & Dmitry V. Kravchenko

Authors
  1. Yuri F. Drygin
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  2. Anatoly N. Blintsov
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  3. Vitaly G. Grigorenko
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  4. Irina P. Andreeva
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  5. Alexander P. Osipov
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  6. Yuri A. Varitzev
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  7. Alexander I. Uskov
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  8. Dmitry V. Kravchenko
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  9. Joseph G. Atabekov
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Corresponding author

Correspondence to Yuri F. Drygin.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00253-011-3656-x.

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Drygin, Y.F., Blintsov, A.N., Grigorenko, V.G. et al. Highly sensitive field test lateral flow immunodiagnostics of PVX infection. Appl Microbiol Biotechnol 93, 179–189 (2012). https://doi.org/10.1007/s00253-011-3522-x

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  • DOI: https://doi.org/10.1007/s00253-011-3522-x

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