Abstract
The effects of cortex-lysis related genes with the pdaA, sleB, and cwlD mutations of Bacillus subtilis (natto) NAFM5 on sporulation and germination were investigated. Single or double mutations did not prevent normal sporulation, but did affect germination. Germination was severely inhibited by the double mutation of sleB and cwlD. The quality of natto made with the sleB cwlD double mutant was tested, and the amounts of glutamic acid and ammonia were very similar to those in the wild type. The possibility of industrial development of natto containing a reduced number of viable spores is presented.
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Acknowledgements
The authors would like to thank Dr. Keitarou Kimura (National Food Research Institute) for the supply of B. subtilis (natto) NAFM5. This work was supported by Grants-in-Aid for Scientific Research (B) (19380047) and for the Global COE program of the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MECSSTJ).
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Mitsui, N., Murasawa, H. & Sekiguchi, J. Development of natto with germination-defective mutants of Bacillus subtilis (natto). Appl Microbiol Biotechnol 82, 741–748 (2009). https://doi.org/10.1007/s00253-009-1894-y
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DOI: https://doi.org/10.1007/s00253-009-1894-y