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Application of Plackett–Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16

  • Biotechnological Products and Process Engineering
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Abstract

The objective of this study was to use statistically based experimental designs for the optimization of xylanase production from Alternaria mali ND-16. Ten components in the medium were screened for nutritional requirements. Three nutritional components, including NH4Cl, urea, and MgSO4, were identified to significantly affect the xylanase production by using the Plackett–Burman experimental design. These three major components were subsequently optimized using the Doehlert experimental design. By using response surface methodology and canonical analysis, the optimal concentrations for xylanase production were: NH4Cl 11.34 g L−1, urea 1.26 g L−1, and MgSO4 0.98 g L−1. Under these optimal conditions, the xylanase activity from A. mali ND-16 reached 30.35 U mL−1. Verification of the optimization showed that xylanase production of 31.26 U mL−1 was achieved.

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Authors and Affiliations

  1. Department of Plant Science, North Dakota State University, Fargo, 58105, USA

    Yin Li

  2. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Zhiqiang Liu

  3. NSF I/UCRC for Biocatalysis and Bioprocessing of Macromolecules, Six Metro Tech Center, Brooklyn, New York, NY, 11201, USA

    Zhiqiang Liu

  4. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 2120013, People’s Republic of China

    Fengjie Cui

  5. Department of Cereal and Food Science, North Dakota State University, Fargo, 58105, USA

    Zhisheng Liu

  6. Department of Statistics, North Dakota State University, Fargo, 58105, USA

    Hui Zhao

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  1. Yin Li
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  2. Zhiqiang Liu
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  3. Fengjie Cui
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  4. Zhisheng Liu
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Correspondence to Yin Li.

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Li, Y., Liu, Z., Cui, F. et al. Application of Plackett–Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16. Appl Microbiol Biotechnol 77, 285–291 (2007). https://doi.org/10.1007/s00253-007-1167-6

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  • DOI: https://doi.org/10.1007/s00253-007-1167-6

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