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Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile

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Abstract

In this study, the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans. In this fermentation process, this device was used for the characterization of the oxidation pattern of different strains. G. oxydans NCIMB 8084 forms 2,5-diketogluconate from d-glucose in a multi-stage process via three different membrane-bound dehydrogenases. This strain was chosen for a scale-up of the process from shaking-flasks to a 2-l stirred vessel. An enhancement of 2,5-diketogluconate production was realized by controlling the pH at different levels during the fermentation.

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Acknowledgements

The authors kindly thank Prof. H. Sahm and S. Bringer-Meyer from the Institute of Biotechnology at the Jülich Research Center, Germany, for their support of our work and for providing G. oxydans strain DSM 3503 for this study. The authors ensured that all experiments comply with the current German laws.

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Authors and Affiliations

  1. Department of Biochemical Engineering, Aachen University of Technology, Worringer Weg 1, 52056, Aachen, Germany

    M. Silberbach, B. Maier & J. Büchs

  2. Department of Microbiology, Aachen University of Technology, Worringer Weg 1, 52056, Aachen, Germany

    M. Zimmermann

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  1. M. Silberbach
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  2. B. Maier
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  3. M. Zimmermann
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  4. J. Büchs
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Silberbach, M., Maier, B., Zimmermann, M. et al. Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile. Appl Microbiol Biotechnol 62, 92–98 (2003). https://doi.org/10.1007/s00253-003-1222-x

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  • DOI: https://doi.org/10.1007/s00253-003-1222-x

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