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Influence of threonine exporters on threonine production in Escherichia coli

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Abstract.

Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine-resistant phenotype when overexpressed, and they decrease the accumulation of radioactive metabolites derived from [14C] L-threonine. The evidence presented supports the conclusion that both RhtB and RhtC catalyze efflux of L-threonine and other structurally related neutral amino acids, but that the specificities of these two carriers differ substantially.

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Authors and Affiliations

  1. Institut für Biochemie der Universität zu Köln, Zülpicherstrasse 47, 50674 Cologne, Germany, , , , ,

    D. Kruse,  R. Krämer &  A. Burkovski

  2. Institut für Biotechnologie 1, Forschungszentrum Jülich, 52425 Jülich, Germany, , , , ,

    L. Eggeling

  3. Degussa., R & D Feed Additives/Biotechnology, P.O. Box 1112, 33788 Halle, Germany, , , , ,

    M. Rieping &  W. Pfefferle

  4. Division of Biology, University of California at San Diego, La Jolla, CA 92093–0116, USA, , , , ,

    J. Tchieu,  Y. Chung &  M. Saier

Authors
  1. D. Kruse
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  2. R. Krämer
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  3. L. Eggeling
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  4. M. Rieping
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  5. W. Pfefferle
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  6. J. Tchieu
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  7. Y. Chung
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  8. M. Saier
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  9. A. Burkovski
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Kruse, .D., Krämer, .R., Eggeling, .L. et al. Influence of threonine exporters on threonine production in Escherichia coli . Appl Microbiol Biotechnol 59, 205–210 (2002). https://doi.org/10.1007/s00253-002-0987-7

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  • DOI: https://doi.org/10.1007/s00253-002-0987-7

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