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Influence of Bacterial Lysate Quality on Growth of Two Bacterioplankton Species

  • Environmental Microbiology
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Abstract

All physico-chemical parameters that affect bacterial growth rate will also affect bacterial molecular composition, which in turn influences the chemical composition of bacterial lysate and its turnover rate in the ecosystem. To produce qualitatively different lysates, Vibrio sp. cells were grown under different pH, salt, or temperature conditions in rich growth media and then washed and lysed by autoclaving. Both the absolute concentrations and the ratios between elements in the lysates varied with different growth conditions, implying differences in lysate quality. Either Pseudoalteromonas sp. or Vibrio sp. was grown on the lysates at non-limiting lysate concentrations. Different lysates supported growth rates of Pseudoalteromonas sp. in the range from 0.25 to 1.53 h−1. On the other hand, growth rates of Vibrio sp. grown on its own lysates were around 0.4 h−1 and were not dependent on lysate quality. Two orders of magnitude decrease in Zn concentration in Vibrio sp. cells grown on different lysates as compared to cells grown on rich growth medium suggested that Zn might be a factor limiting growth. In the simple microbial loop studied, the initial difference in lysate quality was preserved in Pseudoalteromonas sp., whereas Vibrio sp. decreased the initial differences in lysate quality, thereby neutralizing the primary effect of environmental conditions on carbon turnover.

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Acknowledgment

This work was supported by the Slovenian Research Agency (ARRS 3311-02-831488).

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Authors and Affiliations

  1. Microbiology, Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia

    Duško Odić, Ines Mandić-Mulec & David Stopar

  2. Analytical Chemistry Laboratory, National Institute of Chemistry Slovenia, Hajdrihova 19, 1001, Ljubljana, Slovenia

    Bojan Budič

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  1. Duško Odić
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  2. Bojan Budič
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  3. Ines Mandić-Mulec
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  4. David Stopar
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Correspondence to David Stopar.

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Odić, D., Budič, B., Mandić-Mulec, I. et al. Influence of Bacterial Lysate Quality on Growth of Two Bacterioplankton Species. Microb Ecol 59, 246–252 (2010). https://doi.org/10.1007/s00248-009-9557-1

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  • DOI: https://doi.org/10.1007/s00248-009-9557-1

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