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The Occurrence of Multidrug-Resistant Pseudomonas aeruginosa on Hydrocarbon-Contaminated Sites

  • Environmental Microbiology
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Abstract

The main aim of this paper was the comprehensive estimation of the occurrence rate and the antibiotic-resistance conditions of opportunistic pathogen Pseudomonas aeruginosa in hydrocarbon-contaminated environments. From 2002 to 2007, 26 hydrocarbon-contaminated sites of Hungary were screened for the detection of environmental isolates. Altogether, 156 samples were collected and examined for the determination of appearance, representative cell counts, and antibiotic-resistance features of P. aeruginosa. The detected levels of minimal inhibitory concentrations of ten different drugs against 36 environmental strains were compared to the results of a widely used reference strain ATCC 27853 and four other clinical isolates of P. aeruginosa. Based on our long-term experiment, it can be established that species P. aeruginosa was detectable in case of 61.5% of the investigated hydrocarbon-contaminated sites and 35.2% of the examined samples that shows its widespread occurrence in polluted soil–groundwater systems. In the course of the antibiotic-resistance assay, our results determined that 11 of the examined 36 environmental strains had multiple drug-resistance against several clinically effective antimicrobial classes: cephalosporins, wide spectrum penicillins, carbapenems, fluoroquinolones, and aminoglycosides. The fact that these multiresistant strains were isolated from 8 different hydrocarbon-contaminated sites, mainly from outskirts, confirms that multiple drug-resistance of P. aeruginosa is widespread not only in clinical, but also in natural surroundings as well.

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Acknowledgments

This research work was supported by the Ányos Jedlik Programme (OM 00120/2007) and Regional University Center of Excellence in Environmental Industry Based on Natural Resources, Szent István University (PÁZMÁNY, RET-12/2005).

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Authors and Affiliations

  1. Department of Environmental Protection and Environmental Safety, Szent István University, 1 Páter Károly Street, 2103, Gödöllő, Hungary

    Balázs Kriszt, Béla Atzél, Gabriella Szabó, István Szabó & Sándor Szoboszlay

  2. Regional University Center of Excellence, Szent István University, 1 Páter Károly Street, 2103, Gödöllő, Hungary

    Edit Kaszab & Péter Harkai

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  1. Edit Kaszab
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  2. Balázs Kriszt
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  3. Béla Atzél
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  4. Gabriella Szabó
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  5. István Szabó
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  7. Sándor Szoboszlay
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Correspondence to Balázs Kriszt.

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Kaszab, E., Kriszt, B., Atzél, B. et al. The Occurrence of Multidrug-Resistant Pseudomonas aeruginosa on Hydrocarbon-Contaminated Sites. Microb Ecol 59, 37–45 (2010). https://doi.org/10.1007/s00248-009-9551-7

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  • DOI: https://doi.org/10.1007/s00248-009-9551-7

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