Abstract.
ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance of swelling-activated chloride (Cl−) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance of a Cl− conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support the hypothesis that ClC-2 is capable of mediating regulatory volume decrease.
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Received: 12 August/Revised: 23 October 1998
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Xiong, H., Li, C., Garami, E. et al. ClC-2 Activation Modulates Regulatory Volume Decrease. J. Membrane Biol. 167, 215–221 (1999). https://doi.org/10.1007/s002329900485
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DOI: https://doi.org/10.1007/s002329900485