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Colchicine Modulates Oxidative Stress in Serum and Leucocytes from Remission Patients with Family Mediterranean Fever Through Regulation of Ca2+ Release and the Antioxidant System

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Abstract

We investigated the effects of colchicine on oxidative stress and Ca2+ release in serum and polymorphonuclear leucocytes (PMNs) of Familial Mediterranean Fever (FMF) patients with attack, remission and unremission periods. Eighteen FMF patients and six age-matched healthy subjects in four groups were used. The first group was a control. The second group included patients with active FMF. The third and fourth groups were patients with remission and unremission, respectively. Colchicine (1.5 mg/day) was given to the third and fourth groups for 1 month. PMN cells, serum lipid peroxidation and intracellular Ca2+-release levels in the attack and unremission groups were higher than in those in controls, although they were lower in the remission group than in the attack group. Serum vitamin E and β-carotene concentrations were higher in the remission group than in the control and attack groups. However, PMN, serum lipid peroxidation and Ca2+-release levels were further increased in the unremission group compared to the attack group. Glutathione peroxidase, reduced glutathione and vitamin A values in the four groups did not change by FMF and colchicine. In conclusion, we observed that colchicine induced protective effects on oxidative stress by modulating vitamin E, β-carotene and Ca2+-release levels in FMF patients with a remission period.

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Acknowledgments

The study was partially supported by the Scientific Research Unit of Suleyman Demirel University (protocol 1789-TU-09). An abstract of the study as a poster presentation was submitted at the 3rd International Congress on Cell Membranes and Oxidative Stress Focus on Calcium Signaling and TRP Channels, June 22–27, 2010, Isparta, Turkey (www.cmos.org.tr).

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The authors declare that there are no conflicts of interest.

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Correspondence to Mustafa Nazıroğlu.

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Şahin, M., Cihangir Uğuz, A., Demirkan, H. et al. Colchicine Modulates Oxidative Stress in Serum and Leucocytes from Remission Patients with Family Mediterranean Fever Through Regulation of Ca2+ Release and the Antioxidant System. J Membrane Biol 240, 55–62 (2011). https://doi.org/10.1007/s00232-011-9342-1

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