Abstract
Three major curcuminoids (I, II and III) were purified from turmeric and tested for their ability to modulate the function of P-glycoprotein ATPase of the insecticide-resistant pest Helicoverpa armigera (Ha-Pgp). The curcumin mixture inhibited the activity of Ha-Pgp ATPase by 80–90% at 100 μM concentration. Along with curcuminoids I, II and III, it inhibited the verapamil- and ethylparaoxon-stimulated Ha-Pgp ATPase activity. Curcuminoid binding was quantitated by quenching the intrinsic Trp fluorescence of purified Ha-Pgp ATPase. Transport was monitored in proteoliposomes containing Ha-Pgp ATPase using the high-affinity fluorescent substrate tetramethylrosamine (TMR) in real time. Addition of the curcuminoid mixture collapsed the TMR concentration gradient generated by Ha-Pgp ATPase. Inhibition studies on Ha-Pgp ATPase activity are important to develop strategies to overcome insecticide resistance in this pest.
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Acknowledgments
This research was supported by a research grant to K. S. from the Department of Science and Technology and SAP of the University Grant Commission, New Delhi, India. R. M. A. thanks the Council of Scientific and Industrial Research, New Delhi, India, for financial assistance in the form of SRF during this work.
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Aurade, R.M., Jayalakshmi, S.K. & Sreeramulu, K. Modulatory Effects of Natural Curcuminoids on P-Glycoprotein ATPase of Insecticide-Resistant Pest Helicoverpa armigera (Lepidopetera: Noctüidae). J Membrane Biol 236, 271–278 (2010). https://doi.org/10.1007/s00232-010-9299-5
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DOI: https://doi.org/10.1007/s00232-010-9299-5