Abstract
We demonstrated previously that the hypothalamic supraoptic nucleus (SON) undergoes an axonal sprouting response following a unilateral lesion of the hypothalamo-neurohypophysial tract in a 35-day-old rat to repopulate the partially denervated neural lobe (NL). However, no sprouting occurs following the same injury in a 125-day-old rat. We previously reported a significant increase in Thy-1 protein in the SON of a 125-day-old rat compared to a 35-day-old rat in the absence of injury. Thy-1 is a cell surface glycoprotein shown to inhibit axonal outgrowth following injury; however, we did not look at axotomy’s effect on Thy-1 in the SON. Therefore, we sought to determine the integrin ligands that bind Thy-1 in the SON and how axotomy impacts Thy-1. Like what others have shown, the co-immunoprecipitation analysis demonstrated that Thy-1 interacts with αvß3 and αvß5 integrin dimers in the SON. We used western blot analysis to examine protein levels of Thy-1 and integrin subunits following injury in the 35- and 125-day-old rat SON and NL. Our results demonstrated that Thy-1 protein levels increase in the lesion SON in a 35-day-old rat. The quantitative dual-fluorescent analysis showed that the increase in Thy-1 in the lesion SON occurred in astrocytes. There was no change in Thy-1 or integrin protein levels following injury in the 125-day-old following injury. Furthermore, the axotomy significantly decreased Thy-1 protein levels in the NL of both 35- and 125-day-old rats. These results provide evidence that Thy-1 protein levels are injury dependent in the magnocellular neurosecretory system.
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The datasets generated during the current study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors thank Dr. John Watt at the University of North Dakota for using the Olympus microscope. This material is based upon work supported by the National Science Foundation under Grant No. 1643998, anonymous donors that have financially supported research in the biology department, and the Office of URSCA (Undergraduate Research, Scholarship, and Creative Activity) at Concordia College.
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Askvig, J.M., Irmen, R.E., Dalzell, T.S. et al. Axotomy results in an increase in Thy-1 protein in the 35-day-old rat supraoptic nucleus. Exp Brain Res 241, 851–864 (2023). https://doi.org/10.1007/s00221-023-06562-4
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DOI: https://doi.org/10.1007/s00221-023-06562-4