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Assessing allergenicity of different tomato ecotypes by using pooled sera of allergic subjects: identification of the main allergens

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Abstract

An evaluation of the potential allergenicity of different tomato ecotypes is reported. Twelve tomato ecotypes were assessed through a proteomic approach, using pools of sera of allergic patients from two different regions (Emilia Romagna in Northern Italy and Campania in Southern Italy), in order to identify the major allergens and evaluate differences in IgE binding properties of the tomato cultivars. Pooled sera of allergic people from Emilia Romagna showed as the main allergen a suberization-associated anionic peroxidase, whereas pooled sera of allergic patients from Campania were mostly reactive to profilin. The two proteins were identified through a proteomic approach based on the use of high-resolution mass spectrometric techniques. Quite interestingly, in some cases, several ecotypes showed a less reactivity toward patients’ sera than other, potentially indicating the possibility to identify ipoallergenic varieties. Anyway, the allergenic pattern response to tomatoes was serum-specific, indicating that the allergenic properties of different tomato ecotypes are defined by the specific proteins to which the patient is sensitized, a strong indication that ipoallergenicity of the different ecotypes is possible, but mostly related to the individual susceptibility.

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Authors and Affiliations

  1. Dipartimento di Chimica Organica e Industriale, University of Parma, Parco Area delle Scienze 17/A, 43125, Parma, Italy

    Mariangela Bencivenni, Chiara Bottesini, Arnaldo Dossena, Rosangela Marchelli & Stefano Sforza

  2. Centro Interdipartimentale Misure ‘Giuseppe Casnati’, University of Parma, Parco Area delle Scienze 23/A, 43125, Parma, Italy

    Andrea Faccini

  3. Dipartimento di Scienze del Suolo, della Pianta, dell’Ambiente e delle Produzioni Animali, University of Naples, Reggia di Portici—Via Università 100, 80055, Portici-Naples, Italy

    Rosa Rao

  4. Dipartimento di Medicina Clinica e Scienze Cardiovascolari e Immunologiche, University of Naples, Naples, Italy

    Aikaterini Detoraki & Gianni Marone

  5. Dipartimento di Scienze Cliniche, University of Parma, Via Gramsci 14, 43100, Parma, Italy

    Erminia Ridolo

  6. Dipartimento di Clinica Medica, Nefrologia e Scienze della Prevenzione, Via Gramsci 14, 43100, Parma, Italy

    Pier Paolo Dall’Aglio

Authors
  1. Mariangela Bencivenni
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  2. Andrea Faccini
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  3. Chiara Bottesini
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  4. Rosa Rao
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  5. Aikaterini Detoraki
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  6. Erminia Ridolo
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  7. Gianni Marone
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  8. Pier Paolo Dall’Aglio
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  9. Arnaldo Dossena
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  10. Rosangela Marchelli
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  11. Stefano Sforza
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Corresponding author

Correspondence to Stefano Sforza.

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Bencivenni, M., Faccini, A., Bottesini, C. et al. Assessing allergenicity of different tomato ecotypes by using pooled sera of allergic subjects: identification of the main allergens. Eur Food Res Technol 234, 405–414 (2012). https://doi.org/10.1007/s00217-011-1640-4

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  • DOI: https://doi.org/10.1007/s00217-011-1640-4

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