Abstract
Thermal texture evolution kinetics (90–110 °C) of nonpretreated and high-pressure pretreated (HP = 400 MPa, 60 °C, 15 min) carrots were determined using a multiparameter approach (cutting, compression). Alcohol Insoluble Residues (AIR) were extracted before and after thermal processing of the samples and the degree of methylation (DM) was estimated. The β-elimination kinetics of the water soluble pectin extract from the AIR was studied and related to the changes in material properties. Morphological changes and tissue-failure characteristics were monitored. The mechanical properties were strongly dependent on the processing temperature and the DM of the samples. Texture degradation rate constants were independent of the texture measurement method. Increasing temperature accelerated the β-elimination reaction (k b) and the thermosoftening (k x) rates, but pretreatment condition slowed down the rates. Interestingly, a strong correlation (r > 0.99) between k b and k x occurred. Thermal processing resulted in cell-wall thickening accompanied by a transition from cell rupture to cell separation, a process retarded by pretreatment condition.
Similar content being viewed by others
Abbreviations
- β:
-
Beta
- ∞:
-
Infinity
- ø:
-
Diameter
- A0 :
-
Initial concentration of galacturonides (mM/g AIR)
- At :
-
Concentration of galacturonides after a given time (mM/g AIR)
- Ea :
-
Activation energy (kJ mol−1)
- H:
-
Hardness (g)
- Ho, Ht :
-
Reference hardness value [initial, at time t] (g)
- H∞ :
-
Hardness after a long time (g)
- kx :
-
Texture degradation rate constant (min−1)
- kb :
-
β-elimination reaction rate constant (mM min−1/g AIR)
- kxref :
-
Reference texture degradation rate constant (min−1)
- r:
-
Correlation coefficient
- R:
-
Universal gas constant [8.314] (J mol−1 K−1)
- R2 :
-
Regression coefficient
- t:
-
Time (min)
- T:
-
Temperature (K)
- Tc :
-
Constant temperature (K)
- T0, Tref :
-
Reference absolute temperature (K)
- AIR:
-
Alcohol insoluble residue
- DM:
-
Degree of methylation
- EU:
-
European Union
- PME:
-
Pectinmethylesterase
- SAS:
-
Statistical software
References
Andersson A, Gekas V, Lind I, Oliveira F, Oste R (1994) Effects of preheating on potato texture. Crit Rev Food Sci Nutr 34:229–251
Harker F, Redgwell R, Hallet I, Murray S (1997) Texture of fresh fruit. Hort Rev 20:121–124
Van Buren J (1974) Heat treatments and the texture of restart cherries. J Food Sci 39:1203–1205
Duvetter T, Fraeye I, Van Hoang T, Van Buggenhout S, Verlent I, Smout C, VanLoey A, Hendrickx M (2005) Effect of pectinmethylesterase infusion methods and processing techniques on strawberry firmness. J. Food Sci 70(6):S383–S388
Smout C, Sila D, Truong V, VanLoey A, Hendrickx M (2005) Effect of preheating and calcium pretreatment on pectin structure and thermal texture degradation: A case study on carrots. J Food Eng 67: 419–425
Sila D, Smout C, Truong V, VanLoey A, Hendrickx M (2005) The influence of pretreatment conditions on the texture and cell wall components of carrots during thermal processing. J Food Sci 70(2):E85–E91
Van Buren J (1973) Improve firmness without additives. Food Eng 45:127
Steinbuch E (1976) Technical note: Improvement of texture of frozen vegetables by stepwise blanching treatments. J Food Technol 11:313–316
Siliha H, Giershner K (1995) Effect of heat treatments on cell wall polysaccharides of canned carrots. In: Omran H, Buckenhuskes H (eds) Hydrocolloids in foodprocessing. Proceedings of the international conference, Suez Canal University, Ismailia, Egypt, 29 Nov–1 Dec 1994. Suez Canal University Press, Ismailia, pp 13–36
Thakur B, Singh K, Handa K (1997) Chemistry and uses of pectin—A review. Crit Rev Food Sci Nutr 37:47–73
Albersheim P, Neukom H, Deuel H (1960) Splitting of pectin chain molecules in neutral solution. Arch Biochem Biophy 90:46–51
Krall S, McFeeters R (1998) Pectin hydrolysis: Effect of temperature, degree of methylation, pH and calcium on hydrolysis rates. J Agric Food Chem 46:1311–1315
Sila D, Smout C, Elliot F, Van Loey A, Hendrickx M (2006) Nonenzymatic depolymerization of carrots pectin: Towards a better understanding of carrot texture during thermal processing. J Food Sci 71(1):E001–E007
Jackman R, Stanley D (1995) Perspective in textural evaluation of plant foods. Trends Food Sci Tech 6:187–194
Nielsen M, Maartens H, Kaack K (1998) Low frequency utrasonic texture measurements in carrots (Daucus carota L.) in relation to water loss and storage. Postharvest Biol Tech 14:297–308
Rojas A, Gerschenson L, Marangoni A (2001) Contributions of cellular components on the rheological behaviour of kiwi fruit. Food Res Intl 34:189–195
Mc Feeters R, Armstrong S (1984) Measurement of pectin methylation in plant cell walls. Anal Biochem 139:212–217
Blumenkrantz N, Asboe-Hansen G (1973) New methods for the determination of uronic acids. Anal Biochem 54:484–489
Klavons J, Bennett R (1986) Determination of methanol using alcohol oxidase and its application to methyl ester content of pectins. J Food Chem 34:597–599
Braga M, Pessoni R, Dietrich S (1998) Cell wall polysaccharide composition of leaves of tropical rubiaceae differing in phytoalexin response. Rev Bras Fisiol Vegetal 10(2):71–78
Kravtchenko T, Arnould I, Voragen A, Pilnik W (1992) Improvement of the selective depolymerization of pectic substances by chemical beta-elimination in aqueous solution. Carbohyd Polym 19:237–242
Rizvi F, Tong C (1997) Fractional conversion for determining texture degradation kinetics of vegetables. J Food Sci 62(1):1–7
SAS Institute (2004) SAS user’s guide. Statistics. 9th Ed. Cary, NC, pp 1–683
Sajjanantakul T, Van Buren J, Downing D (1989) Effect of methyl ester content on the heat degradation of chelator soluble carrot pectin. J Food Sci 54:1272–1277
Sila D, Smout C, Truong V, Hendrickx M (2004) Effects of high pressure pretreatment and calcium soaking on the texture degradation kinetics of carrots during thermal processing. J Food Sci 69(5):205–211
Sila D, Smout C, Satara Y, Truong V, VanLoey A, Hendrickx M (2007) Combined thermal and high pressure effect on carrot pectinmethylesterase stability and catalytic activity. J Food Eng 78(3):755–764
Cano M, Hernandez A, Ancos B (1997) High pressure and temperature effects on enzyme inactivation in strawberry and orange products. J Food Sci 62(1):85–88
Van Den Broeck L (2000) Kinetics of temperature and pressure inactivation of pectinmethylesterase from orange and tomatoes. Ph.D. Dissertation, Faculteit landbouwkundige en Toegepaste Biologische Wetenschappen, Katholieke Universiteit, Leuven, Belgium
Verlent I, Van Loey A, Smout C, Duvetter T, Ly Nguyen B, Hendrickx M (2004) Changes in purified tomato pectinmethylesterase activity during thermal and high pressure treatment. J Sci Food Agric 84:1839–1847
Kiss J (1974) Beta-eliminative degradation of carbohydrates containing uronic acid residues. Adv Carbohyd Chem BI 29:229–233
Warren D, Woodman J (1974) The texture of cooked potatoes: A review. J Sci Food Agric 25:129–138
Lecain S, Ng A, Parker M, Smith A, Waldron K (1997) Modifsication of cell wall polymers of onion waste: Part I. Effect of pressure cooking. Carbohyd Polym 38:59–67
Redgwell R, MacRae E, Hallert I, Fischer P, Perry J, Harker R (1997) In vivo and in vitro swelling of cell walls during fruit ripening. Planta 203:162–173
Acknowledgement
The authors sincerely thank the Research Council and the Interfaculty Board for Development Cooperation of K.U. Leuven, and the Fund for Scientific Research, Flanders (FWO) for financially supporting this research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sila, D.N., Yue, X., VanBuggenhout, S. et al. The relation between (bio-)chemical, morphological, and mechanical properties of thermally processed carrots as influenced by high-pressure pretreatment condition. Eur Food Res Technol 226, 127–135 (2007). https://doi.org/10.1007/s00217-006-0517-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-006-0517-4