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A near-infrared fluorescent probe with viscosity sensitivity in lysosome for cancer visualization

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Abstract

A viscosity-sensitive, lysosome-targeted near-infrared fluorescent probe (PYATT) was reported in this paper. The fluorescent spectra of PYATT are strongly dependent on viscosity, resulting in a Stokes shift of about 190 nm. Given its photostability, low cytotoxicity, and high fluorescence quantum yield, PYATT is expected to be used in cell imaging. Due to the higher viscosity of tumor cells than normal cells, the fluorescence intensity of PYATT in tumor cells is higher than normal cells, which can realize the visualization of tumors. The near-infrared probe (PYATT) is viscosity-dependent in lysosomes, which is valuable in early diagnosis and treatment of tumor.

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Funding

This work was financially supported by the Henan Provincial Science and Technology Research Project of China (No. 232102310369).

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Authors and Affiliations

  1. Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Molecular Sciences, Henan University, Kaifeng, 475004, People’s Republic of China

    Yong Wang, Lixian Fu, Yiyun Tan & Yue Ding

  2. School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of China

    Weixia Qing

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  1. Yong Wang
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Contributions

Y.W.: supervision, conceptualization, and project administration. L.F.: methodology and formal analysis. Y.T.: formal analysis and validation. Y.D.: software. W.Q.: writing, review and editing.

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Correspondence to Weixia Qing.

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Wang, Y., Fu, L., Tan, Y. et al. A near-infrared fluorescent probe with viscosity sensitivity in lysosome for cancer visualization. Anal Bioanal Chem 416, 341–348 (2024). https://doi.org/10.1007/s00216-023-05050-6

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  • DOI: https://doi.org/10.1007/s00216-023-05050-6

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