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Graphene oxide composites for magnetic solid-phase extraction of twelve quinolones in water samples followed by MALDI-TOF MS

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Abstract

Antibiotic compounds in natural waters are normally present at low concentrations. In this paper, an easy and highly sensitive screening method using graphene oxide–functionalized magnetic composites (GO@NH2@Fe3O4) combined with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was established for twelve quinolone antibiotics. GO@NH2@Fe3O4 composites were utilized as adsorbents for magnetic solid-phase extraction. This method combines the advantages of magnetic solid-phase extraction and MALDI-TOF MS, which allows for fast detection of quinolones at low concentrations. To improve absorption efficiency, the following parameters were individually optimized: sample acidity, extraction time, amount of adsorbent used, eluent used, and desorption time. Under the optimum conditions, the established method gave a low detection limit of 0.010 mg/L and allowed the high-throughput screening of twelve quinolone antibiotics (enoxacin, norfloxacin, ciprofloxacin, pefloxacin, fleroxacin, gatifloxacin, enrofloxacin, levofloxacin, sparfloxacin, danofloxacin, difloxacin, and lomefloxacin). The proposed method, having an easily prepared sorbent with a high affinity for quinolones and a convenient, high-throughput detection step, has been shown to have merit for the detection of antibiotics in water samples.

Schematic illustration of the (A) preparation of GO@NH2@Fe3O4 and (B) operating procedure for the MSPE and MALDI-TOF MS detection of QNs

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Funding

This work is a part of the National Key R&D Program of China (Nos. 2017YFF0211301 and 2017YFC1601101) and the Tianjin Scientific and Technology Support Program Funding Agency (No. 16YFZCNC00730).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental & Operational Medicine, No. 1 DaLi Road, HePing District, Tianjin, 300050, China

    Hong-zhi Tang, Yong-hui Wang, Shuang Li, Jin Wu, Jun-wen Li, Huan-ying Zhou & Zhi-xian Gao

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  1. Hong-zhi Tang
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  2. Yong-hui Wang
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  4. Jin Wu
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Correspondence to Huan-ying Zhou or Zhi-xian Gao.

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Tang, Hz., Wang, Yh., Li, S. et al. Graphene oxide composites for magnetic solid-phase extraction of twelve quinolones in water samples followed by MALDI-TOF MS. Anal Bioanal Chem 411, 7039–7049 (2019). https://doi.org/10.1007/s00216-019-02081-w

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