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Assessment of bioavailable B vitamin content in food using in vitro digestibility assay and LC-MS SIDA

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Abstract

Standardized analytical methods, where each B vitamin is extracted from a given sample individually using separate procedures, typically ensure that the extraction conditions provide the maximum recovery of each vitamin. However, in the human gastrointestinal tract (GIT), the extraction conditions are the same for all vitamins. Here, we present an analytically feasible extraction protocol that simulates conditions in the GIT and provides a measure of the content of bioavailable vitamins using LC-MS stable isotope dilution assay. The results show that the activities of both human gastric and duodenal juices were insufficient to liberate absorbable vitamers (AV) from pure cofactors. The use of an intestinal brush border membrane (IBBM) fraction derived from the mucosal tissue of porcine small intestine ensured at least 70% AV recovery. The rate of AV liberation, however, was strongly dependent on the cofactor, e.g., in the case of NADH, it was magnitudes higher than in the case of thiamine diphosphate. For some vitamins in some food matrices, the use of the IBBM fraction assay resulted in lower values for the content of AV than conventional vitamin determination methods. Conventional methods likely overestimate the actual bioavailability of some vitamins in these cases.

Assessment of bioavailable B vitamin content in food

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Funding

Financial support for this research was provided by the European Regional Development Fund (project EU48667) and Estonian Ministry of Education (institutional research funding IUT19-27).

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618, Tallinn, Estonia

    Toomas Paalme & Ildar Nisamedtinov

  2. Center of Food and Fermentation Technologies, Akadeemia tee 15A, 12618, Tallinn, Estonia

    Allan Vilbaste, Kaspar Kevvai, Ildar Nisamedtinov & Kristel Hälvin-Tanilas

  3. Lallemand, Inc., Montreal, Québec, H1N 2C4, Canada

    Ildar Nisamedtinov

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  1. Toomas Paalme
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  2. Allan Vilbaste
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  3. Kaspar Kevvai
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  4. Ildar Nisamedtinov
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  5. Kristel Hälvin-Tanilas
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Correspondence to Kristel Hälvin-Tanilas.

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Paalme, T., Vilbaste, A., Kevvai, K. et al. Assessment of bioavailable B vitamin content in food using in vitro digestibility assay and LC-MS SIDA. Anal Bioanal Chem 409, 6475–6484 (2017). https://doi.org/10.1007/s00216-017-0592-3

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