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Evaluation of sample preparation and chromatographic separation for the parallel determination of taurine and edaravone in rat tissues using HILIC-MS/MS

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Abstract

The quantitative analysis of taurine and edaravone in biological sample is critical in pharmaceutical studies. Although each of them can be individually analyzed by different approaches, concurrent quantification is still a highly challenging task with respect to their great polarity variation and the complex composition of tissue sample. In the present study, to simultaneously determine taurine and edaravone in rat tissue, the sample preparation and chromatographic separation conditions were evaluated and discussed in detail. As for the sample preparation, four kinds of solvent and the volume ratio of the optimal solvent to biological sample were both tested and evaluated based on the chromatographic profile, extraction recovery, and matrix effect (ME). The chromatographic separation was performed in a reverse phase (RP) and two hydrophilic interaction liquid chromatography (HILIC) modes, and the corresponding separation efficiencies were assessed using chromatographic parameters like half-width (W 1/2 ), tailing factor (f t), theoretical plates number (N), and ME. Furthermore, adopted composition of two mobile phase systems and the concentrations of the additives in the optimum buffer system were also investigated on an Atlantis HILIC silica column according to the resultant chromatographic profiles and peak areas of the analytes. The optimal results were obtained when the biological samples were deproteined by 4-fold volume of methanol/acetonitrile (1:3, v/v) and separated on a HILIC column with a gradient elution of acetonitrile/water containing 0.2 % formic acid and 10 mM ammonium formate. The proposed approach was validated and successfully applied to the parallel determination of the tissue distribution of edaravone and taurine in rat tissues.

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References

  1. Yamamoto Y, Kuwahara T, Watanabe K (1996) Antioxidant activity of 3-methyl-1-phenyl-2-pyrazolin-5-one. Redox Rep 2:333–338

    CAS  Google Scholar 

  2. Lukic-Panin V, Deguchi K, Yamashita T, Shang J, Zhang X, Tian F, Liu N, Kawai H, Matsuura T, Abe K (2010) Free radical scavenger edaravone administration protects against tissue plasminogen activator induced oxidative stress and blood brain barrier damage. Curr Neurovasc Res 7:319–329

    Article  CAS  Google Scholar 

  3. Watanabe T, Yuki S, Egawa M, Nishi H (1994) Protective effects of MCI-186 on cerebral ischemia: possible involvement of free radical scavenging and antioxidant actions. J Pharmacol Exp Ther 268:1597–1604

    CAS  Google Scholar 

  4. Yamamoto T, Yuki S, Watanabe T, Mitsuka M, Saito KI, Kogure K (1997) Delayed neuronal death prevented by inhibition of increased hydroxyl radical formation in a transient cerebral ischemia. Brain Res 762:240–242

    Article  CAS  Google Scholar 

  5. Abe K, Yuki S, Kogure K (1988) Strong attenuation of ischemic and post-ischemic brain edema in rats by a novel free radical scavenger. Stroke 19:480–485

    Article  CAS  Google Scholar 

  6. Shinohara Y, Yanagihara T, Abe K, Yoshimine T, Fujinaka T, Chuma T, Ochi F, Nagayama M, Ogawa A, Suzuki N, Katayama Y, Kimura A, Minematsu K (2011) II. Cerebral infarction/transient ischemic attack (TIA). J Stroke Cerebrovasc Dis 20:S31–S73

    Article  Google Scholar 

  7. Guidotti A, Badiani G, Pepeu G (1972) Taurine distribution in cat brain. J Neurochem 19:431–435

    Article  CAS  Google Scholar 

  8. Schaffer SW, Azuma J (1992) Review: myocardial physiological effects of taurine and their significance. Adv Exp Med Biol 315:105–120

    Article  CAS  Google Scholar 

  9. Schaffer S, Solodushko V, Azuma J (2000) Taurine-deficient cardiomyopathy: role of phospholipids, calcium and osmotic stress. Adv Exp Med Biol 483:57–69

    Article  CAS  Google Scholar 

  10. Menzie J, Pan C, Prentice H, Wu JY (2014) Taurine and central nervous system disorders. Amino Acids 46:31–46

    Article  CAS  Google Scholar 

  11. Wu H, Jin Y, Wei J, Jin H, Sha D, Wu JY (2005) Mode of action of taurine as a neuroprotector. Brain Res 1038:123–131

    Article  CAS  Google Scholar 

  12. Chepkova AN, Doreulee N, Yanovsky Y, Mukhopadhyay D, Haas HL, Sergeeva OA (2002) Long-lasting enhancement of corticostriatal neurotransmission by taurine. Eur J Neurosci 16:1523–1530

    Article  CAS  Google Scholar 

  13. Sun M, Xu C (2008) Neuroprotective mechanism of taurine due to up-regulating calpastatin and down-regulating calpain and caspase-3 during focal cerebral ischemia. Cell Mol Neurobiol 28:593–611

    Article  Google Scholar 

  14. Guan W, Zhao Y, Xu C (2011) A combined treatment with taurine and intra-arterial thrombolysis in an embolic model of stroke in rats: increased neuroprotective efficacy and extended therapeutic time window. Transl Stroke Res 2:80–91

    Article  CAS  Google Scholar 

  15. Hao X, Li XL, Liu YB, Shen HT, Yue LQ (2012) Experimental study of the protective effects of edaravone combining with taurine on the cerebral ischemia-reperfusion injury. Chin J Misdiagn 12:1597–1598

    Google Scholar 

  16. Tang DQ, Bian TT, Zheng XX, Li Y, Wu XW, Li YJ, Du Q, Jiang SS (2014) LC-MS/MS methods for the determination of edaravone and/or taurine in rat plasma and its application to a pharmacokinetic study. Biomed Chromatogr 28:1173–1182

    Article  CAS  Google Scholar 

  17. Yu YY, Zheng XX, Bian TT, Li YJ, Wu XW, Yang DZ, Jiang SS, Tang DQ (2013) Development and application of a LC-MS/MS assay for the simultaneous quantification of edaravone and taurine in beagle plasma. J Sep Sci 36:3837–3844

    Article  CAS  Google Scholar 

  18. Cui JF, Liao SH, Sun LN, Yan ZY (2012) Determination of edaravone in rat plasma by an LC-UV method and the application in pharmacokinetic study. Chin Pharm Anal 32:1634–1637

    CAS  Google Scholar 

  19. Gao C, Li X, Li Y, Wang L, Xue M (2010) Pharmacokinetic interaction between puerarin and edaravone, and effect of borneol on the brain distribution kinetics of puerarin in rats. J Pharm Pharmacol 62:360–367

    Article  CAS  Google Scholar 

  20. Sato T, Mizuno K, Ishii F (2008) In vitro metabolism study of edaravone in Wistar and hairless rat skin. Biol Pharm Bull 31:1150–1154

    Article  CAS  Google Scholar 

  21. Mou S, Ding X, Liu Y (2002) Separation methods for taurine analysis in biological samples. J Chromatogr B 781:251–267

    Article  CAS  Google Scholar 

  22. Yan HY, Qiao FX, Tian M, Row KH (2012) Application of 2,4-dinitrofluorobenzene pre-column derivatization to quantitative determination of taurine and its intermediate in beverages and milk samples. J Liq Chromatogr Relat Technol 36:35–43

    Google Scholar 

  23. Wang X, Chi D, Su G, Li L, Shao L (2011) Determination of taurine in biological samples by high-performance liquid chromatography using 4-fluoro-7-nitrobenzofurazan as a derivatizing agent. Biomed Environ Sci 24:537–542

    CAS  Google Scholar 

  24. Zinellu A, Sotgia S, Scanu B, Chessa R, Gaspa L, Franconi F, Deiana L, Carru C (2009) Taurine determination by capillary electrophoresis with laser-induced fluorescence detection: from clinical field to quality food applications. Amino Acids 36:35–41

    Article  CAS  Google Scholar 

  25. Lorenzo MP, Navarrete A, Balderas C, Garcia A (2013) Optimization and validation of a CE-LIF method for amino acid determination in biological samples. J Pharm Biomed Anal 73:116–124

    Article  CAS  Google Scholar 

  26. Huang JM, Weisensee C (2008) Enhancing the determination of sulfonic acids using anion-exchange chromatography with post-column derivatization and spectrometric detection. J Liq Chromatogr Relat Technol 31:509–516

    Article  CAS  Google Scholar 

  27. Huang Y, Tian Y, Zhang Z, Peng C (2012) A HILIC-MS/MS method for the simultaneous determination of seven organic acids in rat urine as biomarkers of exposure to realgar. J Chromatogr B 905:37–42

    Article  CAS  Google Scholar 

  28. Bathena SP, Huang J, Epstein AA, Gendelman HE, Boska MD, Alnouti Y (2012) Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry. J Chromatogr B 893(894):15–20

    Article  Google Scholar 

  29. Gamagedara S, Shi H, Ma Y (2012) Quantitative determination of taurine and related biomarkers in urine by liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem 402:763–770

    Article  CAS  Google Scholar 

  30. Gu LQ, Xin YF, Zhang S, Wen L, Yang SB, Hu XL, Xuan YX (2010) Determination of edaravone in the plasma of beagle dog by LC-MS/MS. Acta Acad Med Zhejiang 2:14–16

    Google Scholar 

  31. Tang DQ, Zheng XX, Li YJ, Bian TT, Yu YY, Du Q, Yang DZ, Jiang SS (2014) Two complementary liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods to study the excretion and metabolic interaction of edaravone and taurine in rats. J Chromatogr B 970:8–17

    Article  CAS  Google Scholar 

  32. Hemstrom P, Irgum K (2006) Hydrophilic interaction chromatography. J Sep Sci 29:1784–1821

    Article  Google Scholar 

  33. US Department of Health and Human Services, Food and Drug Administration, Guidance for Industry (2001), Bioanalytical Method Validation. Available at: http://www.fda.gov/downloads/Drugs/Guidances/ucm070107.pdf. Accessed 30 Nov 2014

  34. Van Eeckhaut A, Lanckmans K, Sarre S, Smolders I, Michotte Y (2009) Validation of bioanalytical LC-MS/MS assays: evaluation of matrix effects. J Chromatogr B 877:2198–2207

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge support for this work sponsored by Qing-Lan and Liu-Da-Ren-Cai-Gao-Feng Project of Jiangsu Province, Jiangsu Overseas Research and Training Program for University Prominent Young and Middle-Aged Teachers and Presidents, and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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    Authors and Affiliations

    1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, 221004, China

      Yin-jie Li, Zheng Li, Xiao-xiao Zheng, Xiao-wen Wu, Shi-rui Wang, Hao Guo, Yan-yan Yu, Meng-zhe Guo, Dong-zhi Yan & Dao-quan Tang

    2. Department of Pharmacy, The First People’s Hospital of Xuzhou, Xuzhou, 221002, China

      Xiao-xiao Zheng

    3. Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical College, Xuzhou, 221004, China

      Yan-yan Yu, Meng-zhe Guo, Dong-zhi Yan & Dao-quan Tang

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    1. Yin-jie Li
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    Correspondence to Dao-quan Tang.

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    Yin-jie Li, Zheng Li and Xiao-xiao Zheng contributed equally to this work.

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    Li, Yj., Li, Z., Zheng, Xx. et al. Evaluation of sample preparation and chromatographic separation for the parallel determination of taurine and edaravone in rat tissues using HILIC-MS/MS. Anal Bioanal Chem 407, 4143–4153 (2015). https://doi.org/10.1007/s00216-015-8635-0

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