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In situ alcoholysis of triacylglycerols by application of switchable-polarity solvents. A new derivatization procedure for the gas-chromatographic analysis of vegetable oils

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Abstract

We describe a new use of switchable-polarity solvents for the simultaneous derivatization and extraction of triacylglycerols from vegetable oils before gas-chromatographic analysis. Different equimolecular mixtures of the commercially available amidine 1,8-diazabicyclo[5.4.0]undec-7-ene and n-alkyl alcohols were tested. Triolein was used as a model compound. Very good results were achieved by using butanol (recovery of butyl oleate was 89 ± 4 %). The procedure was applied for the characterization of the fatty acid profile of different vegetable oils. No statistically significant differences from the results obtained with the application of two traditional methods were evidenced. Moreover, the use of switchable-polarity solvents showed many advantages: owing to the basicity of the amidines, no catalyst was required; the transterification reaction was conducted under mild conditions, one step and in situ; no particular matrix interferences were evidenced; the solvent was recovered.

Switchable polarity solvents perform the simultaneous extraction and transesterification of triacylglycerols from vegetable oils. The method represent a new in situ derivatization procedure for the gas chromatographic characterization of thir fatty acid profiles.

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Acknowledgments

We thank Ilaria Piga Serra, Melody Rose DeSanto, and our students Andrea Bosiso and Javier Gomez for their collaboration. This work was supported by University of Milan-Bicocca Far 2012. Two anonymous reviewers are also thanked for comments and suggestions.

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Authors and Affiliations

  1. Department of Environmental Science, University of Milan-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy

    Francesco Saliu & Marco Orlandi

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  1. Francesco Saliu
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  2. Marco Orlandi
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Correspondence to Francesco Saliu.

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Saliu, F., Orlandi, M. In situ alcoholysis of triacylglycerols by application of switchable-polarity solvents. A new derivatization procedure for the gas-chromatographic analysis of vegetable oils. Anal Bioanal Chem 405, 8677–8684 (2013). https://doi.org/10.1007/s00216-013-7190-9

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  • DOI: https://doi.org/10.1007/s00216-013-7190-9

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