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Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

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Abstract

We explored a fluorescent strategy for sensing ochratoxin A (OTA) by using a single fluorophore-labeled aptamer for detection of OTA. This method relied on the change of the fluorescence intensity of the labeled dye induced by the specific binding of the fluorescent aptamer to OTA. Different fluorescein labeling sites of aptamers were screened, including the internal thymine bases, 3′-end, and 5′-end of the aptamer, and the effect of the labeling on the aptamer affinity was investigated. Some fluorophore-labeled aptamers showed a signal-on or signal-off response. With the fluorescent aptamer switch, simple, rapid, and selective sensing of OTA at nanomolar concentrations was achieved. OTA spiked in diluted red wine could be detected, showing the feasibility of the fluorescent aptamer for a complex matrix. This method shows potential for designing aptamer sensors for other targets.

A simple fluorescent approach for OTA sensing is achieved by using single fluorophore-labeled aptamer. A fluorophore is attached on one site of the aptamer. The affinity binding of OTA induces the alteration of fluorescence properties of the labeled fluorophore as the consequence of the conformation change of the aptamer. OTA can be detected by measuring the change of fluorescence signals of the labeled dye

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (grant No. 21222503), the State Key Laboratory of Environmental Chemistry and Ecotoxicology in the Research Center for Eco-Environmental Sciences of the Chinese Academy of Sciences (grant No. KF2010-24), and the Key Project of Chinese Ministry of Education (grant No. 212020).

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Authors and Affiliations

  1. Research Center for Environmental Science and Engineering, Shanxi University, Taiyuan, 030006, China

    Qiang Zhao & Xia Geng

  2. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Hailin Wang

Authors
  1. Qiang Zhao
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  2. Xia Geng
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  3. Hailin Wang
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Corresponding author

Correspondence to Qiang Zhao.

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Zhao, Q., Geng, X. & Wang, H. Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer. Anal Bioanal Chem 405, 6281–6286 (2013). https://doi.org/10.1007/s00216-013-7047-2

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  • DOI: https://doi.org/10.1007/s00216-013-7047-2

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