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A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome

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Abstract

We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-2H3] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49).

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Acknowledgments

The authors acknowledge financial support from the National Institutes of Health (R01 HL082747 F.S.C., A.H.) and R01 DK069386 (M.J.M.). M.S.B. acknowledges support from the Genome Training Grant (T32 HG000035).

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Authors and Affiliations

  1. Department of Genome Sciences, University of Washington, Seattle, WA, 98195-5502, USA

    Michael S. Bereman, Daniela M. Tomazela & Michael J. MacCoss

  2. Division of Newborn Medicine, Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine/St. Louis Children’s Hospital, St. Louis, MO, 63110, USA

    Hillary S. Heins, Aaron Hamvas & F. Sessions Cole

  3. Department of Pediatrics, University of Padova, 35122, Padova, Italy

    Manuela Simonato

  4. Bambino Gesù Pediatric Hospital, IRCCS, 00165, Rome, Italy

    Paola E. Cogo

  5. Department of Medicine and Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO, 63110, USA

    Bruce W. Patterson

Authors
  1. Michael S. Bereman
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  2. Daniela M. Tomazela
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  3. Hillary S. Heins
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  4. Manuela Simonato
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  5. Paola E. Cogo
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  6. Aaron Hamvas
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  7. Bruce W. Patterson
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  8. F. Sessions Cole
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  9. Michael J. MacCoss
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Corresponding authors

Correspondence to Michael S. Bereman or Michael J. MacCoss.

Additional information

Published in the special issue Young Investigators in Analytical and Bioanalytical Science with guest editors S. Daunert, J. Bettmer, T. Hasegawa, Q. Wang and Y. Wei.

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Bereman, M.S., Tomazela, D.M., Heins, H.S. et al. A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome. Anal Bioanal Chem 403, 2397–2402 (2012). https://doi.org/10.1007/s00216-012-5953-3

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  • DOI: https://doi.org/10.1007/s00216-012-5953-3

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