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Copolymerization of luminol on screen-printed cells for single-use electrochemiluminescent sensors

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Abstract

An efficient electrochemiluminescent (ECL) single-use sensor for H2O2 is presented based on an electropolymerized film prepared on screen-printed gold electrode (gold SPE). A study of the copolymerization of luminol in the presence of different monomers was carried out. The polymeric films were grown potentiodynamically with a potential interval between −0.2 and 1.0 V in 0.2 M H2SO4 and were characterized by their electrochemical, electrochemiluminescent, and superficial features. The polymer with the most efficient growth and ECL emission was poly(luminol-3,3′,5,5′-tetramethylbenzidine) at 1:5 ratio. These prepared SPE cells present good mechanical and photoemissive properties. A semi-logarithmic linearization shows a noticeable four decade-width concentration range with a limit of detection (LOD) of 2.6 × 10−9 M and a precision of 10.2% (n = 5; as relative standard deviation, RSD) in the medium range level. The described SPE ECL sensors will be useful for the determination of oxidase substrates in ECL single-use biosensors.

Schematic diagram of sensor preparation by luminol-TMB copolymer formation for electrochemiluminescent rainwater determination

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Acknowledgments

We acknowledge financial support from the Ministerio de Ciencia e Innovación, Dirección General de Investigación y Gestión del Plan Nacional de I + D + i (Spain; Projects CTQ2009-14428-C02-01 and CTQ2009-14428-C02-02); and the Junta de Andalucía (Proyecto de Excelencia P08-FQM-3535). These projects were partially supported by European Regional Development Funds (ERDF). We greatly thank Dr. Francisco Montilla, from the University of Alicante (Spain), for his helpful comments and Ismael Francisco Díaz and Patricia Salinas for their assistance during the realization of this study.

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Authors and Affiliations

  1. ECSens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, 18071, Granada, Spain

    J. Ballesta-Claver, M. C. Valencia-Mirón & L. F. Capitán-Vallvey

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  1. J. Ballesta-Claver
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  2. M. C. Valencia-Mirón
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  3. L. F. Capitán-Vallvey
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Correspondence to L. F. Capitán-Vallvey.

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Ballesta-Claver, J., Valencia-Mirón, M.C. & Capitán-Vallvey, L.F. Copolymerization of luminol on screen-printed cells for single-use electrochemiluminescent sensors. Anal Bioanal Chem 400, 3041–3051 (2011). https://doi.org/10.1007/s00216-011-4965-8

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  • DOI: https://doi.org/10.1007/s00216-011-4965-8

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