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Detoxification of mercury species—an in vitro study with antidotes in human whole blood

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Abstract

To investigate the effects of mercury species intoxication and to test the efficiency of different commonly applied antidotes, human whole blood and plasma surrogate samples were spiked with inorganic mercury (Hg2+) and methylmercury (MeHg+, CH3Hg+) prior to treatment with the antidotes 2,3-dimercaptopropan-1-ol (British Anti Lewisite), 2,3-dimercaptosuccinic acid (DMSA), and N-acetylcysteine (NAC). For mercury speciation analysis in these samples, liquid chromatography was coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS). Adduct formation between mercury species and physiological thiols (cysteine and glutathione) was observed as well as the release of glutathione under treatment with the antidotes DMSA and NAC.

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Authors and Affiliations

  1. University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149, Münster, Germany

    Stefan Trümpler, Sascha Nowak, Björn Meermann, Wolfgang Buscher, Michael Sperling & Uwe Karst

  2. Environmental Specimen Bank for Human Tissues, University Hospital Münster, Domagkstr. 11, 48149, Münster, Germany

    Gerhard A. Wiesmüller

  3. European Virtual Institute for Speciation Analysis, Mendelstr. 11, 48149, Münster, Germany

    Michael Sperling

Authors
  1. Stefan Trümpler
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  2. Sascha Nowak
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  3. Björn Meermann
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  4. Gerhard A. Wiesmüller
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  5. Wolfgang Buscher
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  6. Michael Sperling
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  7. Uwe Karst
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Correspondence to Uwe Karst.

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Trümpler, S., Nowak, S., Meermann, B. et al. Detoxification of mercury species—an in vitro study with antidotes in human whole blood. Anal Bioanal Chem 395, 1929–1935 (2009). https://doi.org/10.1007/s00216-009-3105-1

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  • DOI: https://doi.org/10.1007/s00216-009-3105-1

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