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Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance

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Abstract

Human immunoglobulin E (hIgE) is such an important protein, because of its involvement in allergic disease, that it is of significance to study the interactions between it and its recognizing elements. In this report an analytical strategy based on surface plasmon resonance (SPR) was developed to probe the pattern of interaction between hIgE and its recognizing molecules, including aptamers and antibodies. The affinity constants of hIgE for the antibody and the aptamer were compared first; the aptamer has more affinity than the antibody for human IgE. To study their pattern of interaction, three different binding approaches, including adding the antibody and the streptavidin-coupled aptamer to the sensing surface, were designed. The results showed that hIgE captured on the sensing surface could form a multivalent complex with the aptamer. An ELISA-like assay using the aptamer as both capture and detection probes was then developed. This work highlights an SPR method for characterizing the interaction between the protein and aptamers that is useful for study of biomolecular interaction patterns and binding properties.

Schematic diagram of the use of surface plasmon resonance for detection of the pattern of interaction of human IgE with its DNA aptamer and antibody

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Acknowledgements

This work was supported by National Natural Foundation of China (Grant No. 20575079) and National Basic Research Program of China (973 Program, No. 2006CB910803).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Key Lab of Analytical Chemistry for Life Science (MOE), Nanjing University, 22 Hankou Road, Nanjing, 210093, China

    Jinli Wang, Jingjuan Xu & Hongyuan Chen

  2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 33 Life Science Park Road, Changping District, Beijing, 102206, China

    Renji Lv & Danke Xu

Authors
  1. Jinli Wang
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  2. Renji Lv
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  3. Jingjuan Xu
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  4. Danke Xu
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  5. Hongyuan Chen
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Corresponding authors

Correspondence to Danke Xu or Hongyuan Chen.

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Wang, J., Lv, R., Xu, J. et al. Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance. Anal Bioanal Chem 390, 1059–1065 (2008). https://doi.org/10.1007/s00216-007-1697-x

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  • DOI: https://doi.org/10.1007/s00216-007-1697-x

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