Abstract
Selenium species were determined using HPLC/ICPMS and HPLC/vapor generation/ICPMS in the urine from seven human volunteers investigated at background selenium concentrations and at slightly elevated concentrations after ingestion of 200 μg Se as a selenite supplement. Trimethylselenonium ion (TMSe) was present, together with selenosugars, in the urine samples, a result that dispels recent doubts about its possible previous misidentification with a cationic selenosugar. Although TMSe was present as only a trace metabolite in urine from five of the seven volunteers (0.02–0.28 μg Se/L, equivalent to 1–5% of the sum of selenosugars and TMSe), it was a significant metabolite (up to 4.6 μg Se/L, 22%) in one volunteer, and it was the major identified metabolite (up to 15 μg Se/L, 53%) in another volunteer. This marked individual variability in the formation of TMSe was maintained in a duplicate investigation of urine from the same seven volunteers.
This is a preview of subscription content, log in via an institution to check access.
References
Rayman MP (2000) Lancet 356:233–241
Francesconi KA, Pannier F (2004) Clin Chem 50:2240–2253
Kobayashi Y, Ogra Y, Ishiwata K, Takayama H, Aimi N, Suzuki KT (2002) Proc Natl Acad Sci USA 99:15932–15936
Gammelgaard B, Madsen KG, Bjerrum J, Bendahl L, Jons O, Olsen J, Sidenius U (2003) J Anal At Spectrom 18:65–70
Gammelgaard B, Bendahl L (2004) J Anal At Spectrom 19:135–142
Bendahl L, Gammelgaard B (2004) J Anal At Spectrom 19:950–957
Gammelgaard B, Bendahl L, Wessel Jacobsen N, Stürup S (2005) J Anal At Spectrom 20:889–893
Kuehnelt D, Kienzl N, Traar P, Le NH, Francesconi KA, Ochi T (2005) Anal Bioanal Chem 383:235–246
Gammelgaard B, Jessen KD, Kristensen FH, Jons O (2000) Anal Chim Acta 404:47–54
Kuehnelt D, Kienzl N, Juresa D, Francesconi KA (2006) J Anal At Spectrom (in press) DOI 10.1039/b607670j
Juresa D, Darrouzes J, Kienzl N, Bueno M, Pannier F, Potin-Gautier M, Francesconi KA, Kuehnelt D (2006) J Anal At Spectrom 21:684–690
Shibata Y, Morita M, Fuwa K (1992) Adv Biophys 28:31–80
Marchante-Gayon JM, Feldmann I, Thomas C, Jakubowski N (2001) J Anal At Spectrom 16:457–463
Acknowledgements
We thank the Austrian Science Fund (FWF Project 16816-N11), the volunteers participating in this study, Pedro Traar for preparing the selenosugar standards, and Margit Geisselbacher for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kuehnelt, D., Juresa, D., Kienzl, N. et al. Marked individual variability in the levels of trimethylselenonium ion in human urine determined by HPLC/ICPMS and HPLC/vapor generation/ICPMS. Anal Bioanal Chem 386, 2207–2212 (2006). https://doi.org/10.1007/s00216-006-0848-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-006-0848-9