Abstract
Multi-analyte detection of environmentally relevant pesticides is performed by using a two-channelled surface plasmon resonance (SPR) biosensor. The special design of the SPR instrument allows the determination of several analytes (DDT, chlorpyrifos and carbaryl) via different immobilization formats. First, simultaneous pesticide monitoring is possible by flowing chlorpyrifos, carbaryl or DDT samples separately over each channel of the SPR system, wherein their corresponding recognition element was previously immobilized. The second approach is based on the multiple and combined immobilization of several analyte recognition elements on the sensing surface of one individual flow cell. In this format, the analysis time for all three pesticides varied from 40 to 60 min depending on the number of regeneration cycles. In most cases, similar detection limits were attained for the target analyte irrespective of the assay format, with sensitivity values at the nanogram per litre level (18–50 ng L−1). The assay reproducibility was proved through the repeated use of the same sensor surface for over more than 200 assay cycles, whereas the absence of biosensor response to non-related analytes showed the specificity and reliability of the analysis. The SPR instrument, including optics, electronics and microfluidics, is already commercialised by the company SENSIA, SL.
Similar content being viewed by others
References
European Environmental Agency (EEA) (1998) Chemicals in the European environment: low doses high stakes. The EEA and UNEP Annual Message 2 on the State of Europes’s Environment
2000/60/EC (2000) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Off J Eur Commun L327 1–72
98/83/EC (1998) Council Directive (98/83/EC) of 3 November 1998 relating to the water quality of water intended for human consumption. Off J Eur Commun L330 32–54
Rowe-Taitt C, Hazzard J, Hoffman KE, Cras JJ, Golden JP, Ligler FS (2000) Biosens Bioelectron 15:579–589
Mastichiadis C, Kabakos E, Christofidis I, Kouparis MA, Winletts C, Misiakos K (2002) Anal Chem 74:6064–6072
Weller MG (2005) Anal Bioanal Chem 381:41–43
Anderson GP, King KD, Gaffney KL, Johnson LH (2000) Biosens Bioelectron 14:771–777
Wadkins RM, Golden JP, Pritsiolas LM, Ligler FS (1997) Biosens Bioelectron 13:407–415
Proll G, Tschmelak J, Gauglitz G (2005) Anal Bioanal Chem 381:61–63
Rodriguez-Mozaz S, Lopez de Alda MJ, Barceló D (2006) Talanta 69:377–384
Homola J, Vaisocherova H, Dostálek J, Piliarik M (2005) Methods 37:26–36
Wegner GJ, Alastair WW, Lee HJ, Corner E, Saeki T, Fang S, Corn RM (2004) Anal Chem 76:5677–5684
O’Brien MJ, Pérez-Luna VH, Brueck SRJ, López GP (2001) Biosens Bioelectron 16:97–108
Mauriz E, Calle A, Manclús JJ, Montoya A, Hildebrandt A, Barceló D, Lechuga LM (2006) Biosens Bioelectron (in press)
Mauriz E, Calle A, Abad A, Montoya A, Hildebrandt A, Barceló D, Lechuga LM (2006) Biosens Bioelectron 21:2129–2136
Mauriz E, Calle A, Manclús JJ, Montoya A, Quitana J, Lechuga LM (2006) Anal Chim Acta 561:40–47
Gonzalez-Martínez MA, Puchades R, Maquieira A (2001) Anal Chem 73:4326–4332
Bienenmann-Ploum M, Korpimäki T, Haasnoot W, Kohen F (2005) Anal Chim Acta 529:115–122
Abad A, Manclús JJ, Mojarrad F, Mercader JV, Miranda MA, Primo J, Guardiola V, Montoya A (1997) J Agr Food Chem 45:3694–3702
Abad A, Primo J, Montoya A (1997) J Agr Food Chem 45:1486–1494
Manclús JJ, Primo J, Montoya A (1996) J Agr Food Chem 44:4052–4062
Grabarek Z, Gergely J (1990) Anal Biochem 185:131–135
Baeumner J (2003) Anal Bioanal Chem 377:434–445
Mauriz E, Calle A, Manclús JJ, Montoya A, Lechuga LM (2006) Sens Actuactors B Chem 118:399–407
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mauriz, E., Calle, A., Manclús, J.J. et al. Multi-analyte SPR immunoassays for environmental biosensing of pesticides. Anal Bioanal Chem 387, 1449–1458 (2007). https://doi.org/10.1007/s00216-006-0800-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-006-0800-z