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Imaging molecular events in single living cells

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Abstract

Fluorescence imaging could be the most powerful technique available for observing spatial and temporal dynamics of biomolecules in living cells, if fluorescent indicators for the relevant biomolecules become available. We have recently developed fluorescent indicators for a variety of second messengers or protein phosphorylations. Using the indicators, we have visualized spatial and temporal dynamics of these molecular events in single living cells. These fluorescent indicators are becoming an indispensable tool for understanding the complex mechanism of signal transduction in living cells.

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Acknowledgements

This work has been supported by JST Japan Science and Technology Agency and grants from the Ministry of Education, Science, and Culture of Japan, the Takeda Science Foundation, and the Sumitomo Foundation.

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Authors and Affiliations

  1. Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Moritoshi Sato

  2. PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan

    Moritoshi Sato

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  1. Moritoshi Sato
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Correspondence to Moritoshi Sato.

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Sato, M. Imaging molecular events in single living cells. Anal Bioanal Chem 386, 435–443 (2006). https://doi.org/10.1007/s00216-006-0716-7

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  • DOI: https://doi.org/10.1007/s00216-006-0716-7

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