Skip to main content
Log in

Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph–photodiode array detector assembly for individual separation–quantification. The optimal extractant (an 80:20 ethanol–water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4a–c

Similar content being viewed by others

References

  1. González-Rodríguez J, Pérez-Juan P, Luque de Castro MD (2004) J Chromatogr 1038:3–9

    Article  Google Scholar 

  2. Visioli F, Poli A, Galli C (2002) Med Res Rev 22(1):65–75

    Article  CAS  Google Scholar 

  3. Ucella N (2001) Trends Food Sci Tech 11:315–327

    Google Scholar 

  4. Angerosa F, D’Alessandro N, Konstantinou P, Di Giacinto L (1995) J Agric Food Chem 43:1802–1807

    Article  CAS  Google Scholar 

  5. Guiso M, Marra C (2005) Nat Prod Res 19(2):105–109

    Article  CAS  Google Scholar 

  6. Mateos R, Espartero JL, Trujillo M, Ríos JJ, León-Camacho M, Alcudia F, Cert A (2001) J Agric Food Chem 49:2185–2192

    Article  CAS  Google Scholar 

  7. Mercier J (1997) Phytochemistry of fruits and vegetables. Clarendon, Oxford, UK

  8. Petrov V, Manolov P (1978) Comp Med East-West 6:123–126

    Article  Google Scholar 

  9. Iwai K, Oi Y, Koyama F, Watanabe K, Hiraoka M, Sekiguchi T (2005) Application: JP 2004-342612 20041126, Jpn Kokai Tokkyo Koho

  10. Bonoli M, Bendini A, Cerretani L, Lercker G, Toschi TG (2004) J Agric Food Chem 52:7026–7032

    Article  CAS  Google Scholar 

  11. Kubo A, Lunde CS, Kubo I (1995) J Agric Food Chem 43:1629–1633

    Article  CAS  Google Scholar 

  12. Trípoli E, Giammanco M, Tabacchi G, Di Majo D, Giammanco S, La Guardia M (2005) Nutr Res Rev 18:98–112

    Google Scholar 

  13. Crea R (2005) Application: WO 2004-US22889 20040714, PCT Int Appl

  14. Beauchamp GK, Keast RSJ, Morel D, Lin J, Pika J, Han Q, Lee CH, Smith AB, Breslin PAS (2005) Nature 437:45–46

    Article  CAS  Google Scholar 

  15. Priego-Capote F, Ruiz-Jiménez J, Luque de Castro MD (2004) J Chromatogr A 1045:239–246

    Article  CAS  Google Scholar 

  16. Savournin C, Baghdikian B, Elias E, Dargouth-Kesraoui F, Boukef K, Balansard G (2001) J Agric Food Chem 49:618–621

    Article  CAS  Google Scholar 

  17. Guinda A, Lanzón A, Ríos JJ, Albi T (2002) Grasas y Aceites 4:419–422

    Google Scholar 

  18. Luque-García JL, Luque de Castro MD (2003) Trends Anal Chem 22:90–98

    Article  Google Scholar 

  19. Luque de Castro MD, Luque-García JL (2002) Acceleration and automation of solid sample treatment. Elsevier, Amsterdam

  20. Chee KK, Wong MK, Lee HK (1996) Anal Chim Acta 330:217–227

    Article  CAS  Google Scholar 

  21. Luque-García JL, Morales-Muñoz S, Luque de Castro MD (2002) Chromatographia 55:117–122

    Article  Google Scholar 

  22. Molins C, Hogendoorn MD, Heusinkveld HAG, Van Harten DC, Van Xoonen P, Baumann RA (1998) Chromatographia 48:450–456

    Article  CAS  Google Scholar 

  23. Stout SJ, DaCunha AR, Allardice DG (1996) Anal Chem 68:653–657

    Article  CAS  Google Scholar 

  24. Zuloaga O, Etxebarría N, Fernández LA, Madariaga JM (1998) Trends Anal Chem 17:642–647

    Article  CAS  Google Scholar 

  25. Luque-García JL, Velasco J, Dobarganes MC, Luque de Castro MD (2004) Food Chem 76:241–248

    Article  Google Scholar 

  26. Silgoner I, Krska R, Lombas E, Gans O, Rosenberg E, Grasserbauer M (1998) Fresenius J Anal Chem 362:120–124

    Article  CAS  Google Scholar 

  27. Cresswell SL, Haswell SJ (1999) Analyst 124:1361–1366

    Article  CAS  Google Scholar 

  28. Canepari S, Cardarelli E, Ghighi S, Scimonelli L (2005) Talanta 66:1122–1130

    Article  CAS  Google Scholar 

  29. Bouaid A, Martín Esteban A, Fernández P, Cámara C (2000) Fresenius J Anal Chem 367:291–294

    Article  CAS  Google Scholar 

  30. Abuín M, Carro AM, Lorenzo RA (2000) J Chromatogr A 889:185–193

    Article  Google Scholar 

  31. Benavente-García O, Castillo J, Lorente J, Ortuño A, Del Río JA (2000) Food Chem 68:457–462

    Article  Google Scholar 

  32. Massart DL, Vanderginste BGM, Buydens LMC, De Jong S, Lewi PJ, Smeyers-Verbeke J (1997) Handbook of chemometrics and qualimetrics, Part A. Elsevier, Amsterdam

Download references

Acknowledgements

The Spanish Comisión Interministerial de Ciencia y Tecnología (CICyT) is gratefully acknowledged for financial support (project No. BQU-2002-13339).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. D. Luque de Castro.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Japón-Luján, R., Luque-Rodríguez, J.M. & Luque de Castro, M.D. Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves. Anal Bioanal Chem 385, 753–759 (2006). https://doi.org/10.1007/s00216-006-0419-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-006-0419-0

Keywords

Navigation