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Specific polyclonal-based immunoassays for sulfathiazole

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Abstract

A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino-N-thiazol-2-yl-benzenesulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL−1 over a measurable range of 0.12–6.71 ng mL−1. Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC50=1.6 ng mL−1) make it a suitable screening method for determination of low levels of STZ in food samples.

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Acknowledgments

This work was supported by project AGL-2000-0522-P4-03 (MCyT, Spain). Nuria Pastor acknowledges a grant from Fundación José y Ana Royo for carrying out Ph.D. studies.

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Authors and Affiliations

  1. Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain

    Nuria Pastor-Navarro, Cristina García-Bover, Ángel Maquieira & Rosa Puchades

Authors
  1. Nuria Pastor-Navarro
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  2. Cristina García-Bover
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  3. Ángel Maquieira
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  4. Rosa Puchades
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Correspondence to Ángel Maquieira or Rosa Puchades.

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Pastor-Navarro, N., García-Bover, C., Maquieira, Á. et al. Specific polyclonal-based immunoassays for sulfathiazole. Anal Bioanal Chem 379, 1088–1099 (2004). https://doi.org/10.1007/s00216-004-2683-1

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