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Biosensors based on enzyme field-effect transistors for determination of some substrates and inhibitors

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Abstract

This paper is a review of the authors' publications concerning the development of biosensors based on enzyme field-effect transistors (ENFETs) for direct substrates or inhibitors analysis. Such biosensors were designed by using immobilised enzymes and ion-selective field-effect transistors (ISFETs). Highly specific, sensitive, simple, fast and cheap determination of different substances renders them as promising tools in medicine, biotechnology, environmental control, agriculture and the food industry.

The biosensors based on ENFETs and direct enzyme analysis for determination of concentrations of different substrates (glucose, urea, penicillin, formaldehyde, creatinine, etc.) have been developed and their laboratory prototypes were fabricated. Improvement of the analytical characteristics of such biosensors may be achieved by using a differential mode of measurement, working solutions with different buffer concentrations and specific agents, negatively or positively charged additional membranes, or genetically modified enzymes. These approaches allow one to decrease the effect of the buffer capacity influence on the sensor response in an aim to increase the sensitivity of the biosensors and to extend their dynamic ranges.

Biosensors for the determination of concentrations of different toxic substances (organophosphorous pesticides, heavy metal ions, hypochlorite, glycoalkaloids, etc.) were designed on the basis of reversible and/or irreversible enzyme inhibition effect(s). The conception of an enzymatic multibiosensor for the determination of different toxic substances based on the enzyme inhibition effect is also described.

We will discuss the respective advantages and disadvantages of biosensors based on the ENFETs developed and also demonstrate their practical application.

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Authors and Affiliations

  1. Laboratory of Biomolecular Electronics, Institute of Molecular Biology & Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143, Kiev, Ukraine

    Sergei V. Dzyadevych, Alexey P. Soldatkin, Yaroslav I. Korpan, Valentyna N. Arkhypova & Anna V. El'skaya

  2. Laboratoire d'Application de la Chimie à l'Environnement, UMR 5634 CNRS-Université Claude Bernard Lyon 1, 43, boulevard du 11 Novembre 1918, 69622, Villeurbanne Cedex, France

    Jean-Marc Chovelon

  3. Laboratoire d'Ingénierie et Fonctionnalisation des Surfaces, FRE 2608, Ecole Centrale de Lyon, 36 avenue Guy de Collongue, 69134, Ecully Cedex, France

    Claude Martelet & Nicole Jaffrezic-Renault

Authors
  1. Sergei V. Dzyadevych
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  2. Alexey P. Soldatkin
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  3. Yaroslav I. Korpan
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  4. Valentyna N. Arkhypova
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  5. Anna V. El'skaya
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  6. Jean-Marc Chovelon
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  7. Claude Martelet
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  8. Nicole Jaffrezic-Renault
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Correspondence to Sergei V. Dzyadevych.

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Dzyadevych, S.V., Soldatkin, A.P., Korpan, Y.I. et al. Biosensors based on enzyme field-effect transistors for determination of some substrates and inhibitors. Anal Bioanal Chem 377, 496–506 (2003). https://doi.org/10.1007/s00216-003-2134-4

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  • DOI: https://doi.org/10.1007/s00216-003-2134-4

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