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Polymorphism in the N-acetyltransferase 1 alleles NAT1*10 and NAT1*14A and cytological gradings of exfoliated urothelial cells in benzidine-exposed Chinese workers: discussion of ethnic differences

  • Metabolic Activation/Inactivation
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Abstract

N-Acetyltransferase 1 (NAT1) modifies bladder cancer risk in European populations exposed to aromatic amines in cigarette smoke. The present study was performed to investigate a possible association between NAT1*10 and NAT1*14A genotypes and bladder cancer risk in benzidine-exposed Chinese workers. Based on the cytological gradings of exfoliated urothelial cells according to Papanicolaou, an exposed research cohort was stratified into subgroups. An allele-specific PCR-based procedure was used to detect the polymorphism in the polyadenylation signal at the locus NAT1 T1088A. A nested PCR-RFLP procedure was conducted to differentiate NAT1*14A (T1088A, C1095A, and G560A) from NAT1*10 (T1088A, C1095A). No significantly different frequencies of homozygous and heterozygous NAT1*10 alleles were found among the subgroups with (i) gradings according to Papanicolaou ≤II (18.3 and 40.2%, respectively), (ii) higher gradings according to Papanicolaou (>II; 28.0 and 34.1%, respectively), and (iii) with bladder cancer (26.3 and 34.2%, respectively). The present data show that NAT1*10 neither displayed an association with an elevated grading of urothelial cells nor a clear impact on the risk for bladder cancer in benzidine-exposed Chinese workers. Discrepancies with the findings in European populations could point to ethnic differences in the disposition of aromatic amines.

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Acknowledgements

This work was supported by Chinese State’s Strategic Basic Research Project (973, grant: 2002CB512900) and German Federal Ministry of Education and Research (BMBF, Project No. CHN-112-99).

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Authors and Affiliations

  1. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 225 Chongqing Rd. (S.), 200025, Shanghai, China

    Wei Chao Guo, Guo Fang Lin & Jian Hua Shen

  2. Municipal Center for Disease Prevention and Control, 200336, Shanghai, China

    Ji Gang Chen

  3. Institute of Occupational Physiology at the University of Dortmund, 44139 , Dortmund, Germany

    Klaus Golka

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  1. Wei Chao Guo
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  2. Guo Fang Lin
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  3. Ji Gang Chen
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  4. Klaus Golka
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  5. Jian Hua Shen
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Correspondence to Jian Hua Shen.

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Guo, W., Lin, G., Chen, J. et al. Polymorphism in the N-acetyltransferase 1 alleles NAT1*10 and NAT1*14A and cytological gradings of exfoliated urothelial cells in benzidine-exposed Chinese workers: discussion of ethnic differences. Arch Toxicol 78, 425–429 (2004). https://doi.org/10.1007/s00204-004-0567-y

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