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A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells

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Abstract

To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection.

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Data availability

The data and material used to support the findings of this study are available from the corresponding author upon request.

References

  • Abubakar A, Malik M, Pebody RG, Elkholy AA, Khan W, Bellos A et al (2016) Burden of acute respiratory disease of epidemic and pandemic potential in the WHO Eastern Mediterranean Region: a literature review. East Mediterr Health J 22(7):513–526

    Article  PubMed  CAS  Google Scholar 

  • Adlhoch C, Fusaro A, Gonzales JL, et al. European Food Safety Authority; European Centre for Disease Prevention and Control; European Union Reference Laboratory for Avian Influenza; Avian influenza overview December 2022–March 2023. EFSA J. 2023; 21(3):e07917.

  • Cameron CM, Cameron MJ, Bermejo-Martin JF et al (2008) Gene expression analysis of host innate immune responses during Lethal H5N1 infection in ferrets. J Virol 82:11308–11317

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chen Y, Liang W, Yang S et al (2013) Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome. Lancet 381:1916–1925

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gao R, Dong L, Dong J, Wen L, Zhang Y, Yu H et al (2010) A systematic molecular pathology study of a laboratory confirmed H5N1 human case. PLoS ONE 5(10):e13315

    Article  PubMed  PubMed Central  Google Scholar 

  • Gu J, Xie Z, Gao Z, Liu J, Korteweg C, Ye J et al (2007) H5N1 infection of the respiratory tract and beyond: a molecular pathology study. Lancet 370(9593):1137–1145

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Herfst S, Schrauwen EJ, Linster M et al (2012) Airborne transmission of influenza A/H5N1 virus between ferrets. Science 336:1534–1541

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Imai M, Watanabe T, Hatta M et al (2012) Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature 486:420–428

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Itoh Y, Shichinohe S, Nakayama M et al (2015) Emergence of H7N9 influenza A virus resistant to neuraminidase inhibitors in nonhuman primates. Antimicrob Agents Chemother 59:4962–4973

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kniss K, Sumner KM, Tastad KJ, Lewis NM, Jansen L, Julian D, et al. Risk for infection in humans after exposure to birds infected with highly pathogenic avian influenza A(H5N1) virus, United States, 2022. Emerg Infect Dis. 2023. https://doi.org/10.3201/eid2906.230103.

  • Li JL, Zhang HX, Feng YM, et al. Identification on biological characteristics of AIV H5N1 monoclonal antibody. Chin J Immunol. 2017;33(3):398–400, 406.

  • Monsalvo AC, Batalle JP, Lopez MF et al (2011) Severe pandemic 2009 H1N1 influenza disease due to pathogenic immune complexes. Nat Med 17:195–199

    Article  PubMed  CAS  Google Scholar 

  • Muramoto Y, Shoemaker JE, Le MQ et al (2014) Disease severity is associated with differential gene expression at the early and late phases of infection in nonhuman primates infected with different H5N1 highly pathogenic avian influenza viruses. J Virol 88:8981–8997

    Article  PubMed  PubMed Central  Google Scholar 

  • Sangsiriwut K, Noisumdaeng P, Uiprasertkul M, Prasertsopon J, Payungporn S, Auewarakul P et al (2021) Full genomic sequences of H5N1 highly pathogenic avian influenza virus in human autopsy specimens reveal genetic variability and adaptive changes for growth in MDCK Cell cultures. Biomed Res Int 22(2021):3890681

    Google Scholar 

  • Vlahos R, Stambas J, Bozinovski S et al (2011) Inhibition of Nox2 oxidase activity ameliorates influenza A virus-induced lung inflammation. PLoS Pathog 7:e1001271

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Walls HH, Harmon MW, Slagle JJ et al (1986) Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses. J Clin Microbial 23(2):240–245

    Article  CAS  Google Scholar 

  • Yatmaz S, Seow HJ, Gualano RC et al (2013) Glutathione peroxidase-1 reduces influenza A virus-induced lung inflammation. Am J Respir Cell Mol Biol 48:17–26

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Kane S, Wu J et al (2009) Mutation-specific antibodies for the detection of EGFR mutations in non-small-cell lung cancer. Clin Cancer Res 15:3023–3028

    Article  PubMed  CAS  Google Scholar 

  • Zhang C (2012) Hybridoma technology for the generation of monoclonal antibodies. Methods Mol Biol 901:117–135

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Liu K, Su Q, Chen X, Wang X, Li Q et al (2022) Clinical features of the first critical case of acute encephalitis caused by the avian influenza A (H5N6) virus. Emerg Microbes Infect 11(1):2437–2446

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zhao N, Wang S, Wang L, Shi Y, Jiang Y, Tseng TJ et al (2023) Epidemiological features and trends in the mortality rates of 10 notifiable respiratory infectious diseases in China from 2004 to 2020: Based on national surveillance. Front Public Health 17(11):1102747

    Article  Google Scholar 

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Acknowledgements

We gratefully acknowledge to Shaanxi Engineering Research Center of Cell Immunology for their generous assistance, as well as to Liting Yan and Xiaoyan Huang for their invaluable cooperation of in the research process.

Funding

This study was supported by the Natural Science Basic Research Program of Shaanxi (Grant No. 2023-JC-QN-0844) and the Key Research and Development Project of Shaanxi (Grant No. 2021ZDLSF01-03).

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Authors and Affiliations

  1. Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People’s Hospital, No. 256 Youyi West Road, Xi’an, 710068, People’s Republic of China

    Haixiang Zhang, Jingying Sun, Yangmeng Feng, Nana Wang, Xiangrong Zhao, Yan Li, Jun Hu, Lijun Sun & Cuixiang Xu

  2. Shaanxi Engineering Research Center of Immunology, Shaanxi Provincial People’s Hospital, Xi’an, 710068, People’s Republic of China

    Haixiang Zhang, Jingying Sun, Yangmeng Feng, Nana Wang, Xiangrong Zhao, Yan Li, Jun Hu, Lijun Sun & Cuixiang Xu

  3. Pharmacy Department, Xi’an No. 3 Hospital, The Affiliated Hospital of Northwest University, Xi’an, 710021, People’s Republic of China

    Jingli Li

Authors
  1. Haixiang Zhang
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  2. Jingying Sun
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  3. Yangmeng Feng
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  4. Jingli Li
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  5. Nana Wang
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  6. Xiangrong Zhao
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  7. Yan Li
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  8. Jun Hu
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  9. Lijun Sun
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  10. Cuixiang Xu
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Contributions

HX Zhang, NN Wang and J Hu designed the experiments. HX Zhang, JY Sun, YM Feng, JL Li, NN Wang, XR Zhao and Y Li conducted the experiments. LJ Sun and CX Xu analyzed the data. HX Zhang, JY Sun and NN Wang wrote the paper. All authors approved the final manuscript.

Corresponding authors

Correspondence to Lijun Sun or Cuixiang Xu.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Ran Wang.

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Zhang, H., Sun, J., Feng, Y. et al. A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells. Arch Microbiol 205, 373 (2023). https://doi.org/10.1007/s00203-023-03693-8

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  • DOI: https://doi.org/10.1007/s00203-023-03693-8

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