Abstract
Bacterial intercellular signaling mediated by small molecules, also called autoinducers (AIs), enables synchronized behavior in response to environmental conditions, and in many bacterial pathogens, intercellular signaling controls virulence gene expression. However, in the intestinal pathogen Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium), although three signals, named AI-1, AI-2 and AI-3, have been described, their roles in virulence remain elusive. AI-3 is the 3,6- isomer of a previously described Vibrio cholerae signaling molecule; 3,5-dimethylpyrazin-2-ol (3,5-DPO). To elucidate the role of AI-3/DPO in S. Typhimurium, we have mapped the global transcriptomic responses to 3,5- and 3,6-DPO isomers in S. Typhimurium. Our studies showed that DPO affects expression of almost 8% of all genes. Specifically, expression of several genes involved in gut-colonization respond to DPO. Interestingly, most of the affected genes are similarly regulated by 3,5-DPO and 3,6-DPO, respectively, indicating that the two isomers have overlapping roles in S. Typhimurium.
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Data availability
The datasets generated during and/or analyzed during the current study are available in Sequence Read Archive under Bioproject accession number PRJNA923488.
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Acknowledgements
This work was supported by a grant from the Novo Nordisk Foundation to Lotte Jelsbak, Grant number NNF19OC0058547.
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This work was supported by a grant from the Novo Nordisk Foundation to Lotte Jelsbak, Grant number NNF19OC0058547.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by CL and LJ. The first draft of the manuscript was written by CL and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Lallement, C., Goldring, W.P.D. & Jelsbak, L. Global transcriptomic response of the AI-3 isomers 3,5-DPO and 3,6-DPO in Salmonella Typhimurium. Arch Microbiol 205, 117 (2023). https://doi.org/10.1007/s00203-023-03450-x
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DOI: https://doi.org/10.1007/s00203-023-03450-x