Abstract
A strictly anaerobic sulfate-reducing strain, designated SG60T, was isolated from paddy soil collected in Fujian Province, China. Growth of strain SG60T was observed at 20–37 °C, pH 5.5–10.0 and 0–0.7% (w/v) NaCl. Strain SG60T showed the highest 16S rRNA sequence similarities to the type strains of Fundidesulfovibrio magnetotacticus FSS-1T (97.2%) and Fundidesulfovibrio putealis DSM 16056T (96.4%). Phylogenetic trees based on the16S rRNA sequence and genome-based phylogenomic tree constructed using 120 core genes showed that strain SG60T clustered with members of the genus Fundidesulfovibrio. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG60T and the most closely related type strain F. magnetotacticus were 78.2% and 21.6%, respectively. Strain SG60T contained MK-7 as the main respiratory quinone and anteiso-C15:0, anteiso-C17:1 ω9c, iso-C16:0 and iso-C16:1 H as the major fatty acids. Strain SG60T produced desulfoviridin and possessed genes (nifHDK) encoding functions involved in nitrogen fixation. The genomic DNA G + C content was 65.5%. Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, strain SG60T represents a novel species of the genus Fundidesulfovibrio, for which the name Fundidesulfovibrio soli sp. nov. is proposed. The type strain is SG60T (= GDMCC 1.3310T = JCM 35676T).
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Data availability
The GenBank accession numbers of strain SG60T are OM850369 (16S rRNA gene) and JAKZKW000000000 (genome), respectively. Other datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- dDDH:
-
Digital DNA–DNA hybridization
- ANI:
-
Average nucleotide identity
- DMSO:
-
Dimethyl sulfoxide
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Acknowledgements
We thank Prof. Aharon Oren for his kindly help and advice on nomenclature.
Funding
This work was supported by National Natural Science Foundation of China (grant no: 42277257 and U21A20295) and the National Science Fund for Excellent Young Scholars of China (grant no: 42222703).
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SGZ and GHL designed the outline. SY drafted the manuscript. SY, RT and CJX performed isolation, deposition and identifications. SY and SH performed the genome analysis. SGZ, CR and GHL revised the manuscript.
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Yang, S., Tang, R., Xie, CJ. et al. A novel sulfate-reducing and nitrogen-fixing bacterium Fundidesulfovibrio soli sp. nov., isolated from paddy soils. Arch Microbiol 205, 80 (2023). https://doi.org/10.1007/s00203-023-03412-3
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DOI: https://doi.org/10.1007/s00203-023-03412-3