Abstract
Despite the worldwide use of 16S rRNA to identify bacterial species, the use of this gene does not discriminate the 750 species in the genus Streptomyces. A MLST scheme was constructed with rpoB, gyrB, recA, trpB and atpD genes to access the genomic variances in Streptomyces species evolution. We analyze the housekeeping genes in 49 Streptomyces isolates from Antarctic soil. It was used two different databases, GenBank and EzBioCloud to compare the 16S sequences. The species founded in both databases are not the same, but in both cases, a few isolates achieve the necessary high percentage to consider the identification. There is a lack of deposited sequences in the other genes, as the data in GenBank proved to be insufficient. Isolate LMA323St_9 has the potential to be studied as a novel species. Besides that, the use of housekeeping genes gives robust phylogenetic information to understand in group relationships.
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Due to the large number of nucleotide sequences, database accession numbers are found in tables throughout the manuscript and in the supplementary data on online resources.
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Acknowledgements
The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for financial support and Prof. Dr. Paris Lavin for the discussions that help the sequences deposit and data analysis.
Funding
This study was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (88882.439723/2019–01).
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MPB and SVDS: work conception. MPB, RSL and APF: data acquisition and analysis. MPB: data interpretation and original draft writing. SVDS: critical revision and supervision.
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Borba, M.P., Ferrero, A.P.d., de Souza Lameira, R. et al. The intricate molecular identification of Streptomyces: a case study on Antarctic soil isolates. Arch Microbiol 204, 476 (2022). https://doi.org/10.1007/s00203-022-03093-4
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DOI: https://doi.org/10.1007/s00203-022-03093-4